V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog A (Avian) (MAFA) (C-Term) antibody

Details for Product No. ABIN152904, Supplier: Log in to see
Antigen
  • RIPE3b1
  • hMafA
  • RGD1562627
  • SMaf1
  • mafl
  • L-MAF
  • MAFL
  • v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian)
  • MAF bZIP transcription factor A
  • v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A
  • v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog Aa
  • Mafa
  • MAFA
  • mafa
  • mafaa
Alternatives
anti-Mouse (Murine) V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog A (Avian) antibody for Chromatin Immunoprecipitation
Epitope
C-Term
37
12
8
5
5
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
70
38
15
3
3
2
2
1
1
1
1
1
Host
Rabbit
70
4
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
69
23
18
18
17
16
16
3
1
Options
Supplier
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Immunogen The immunogen recognized by this antibody maps to a region between residue 300 and the C-terminus (residue 359) of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A using the numbering given in entry NP_919331.1 (GeneID 378435).
Specificity This antibody is specific for MafA protein.
Purification Immunogen affinity purified
Antigen
Alternative Name MafA (MAFA Antibody Abstract)
Background Gene Symbol: MAFA
Gene ID 389692
UniProt Q8CF90
Pathways Hormone Transport
Application Notes Western Blot 1:1000-1:10000, Chromatin Immunoprecipitation 5-15 μg/108 cells, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:1000 to 1:3000, Immunohistochemistry-Frozen 1:10-1:500, Gel Super Shift Assays 1 to 5 μg/20 μL reactionThis antibody can be used for Western blot, electrophoretic mobility shift assays (EMSA), immunohistochemistry, and chromatin immunoprecipitation (ChIP). A band ~36-40 kDa is seen in Western blot analysis. Use in IHC-P was reported in the scientific literature (PMID: 22665223). Other applications have not been tested. *In some cases, the antibody may be diluted further than indicated. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Use in Immunocytochemistry/immunofluorescence and in Immunohistochemistry-Frozen reported in scientific literature (PMID 23967072)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog A (Avian) (MAFA) (C-Term) antibody (ABIN152904) Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 u...
Western Blotting (WB) image for anti-V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog A (Avian) (MAFA) (C-Term) antibody (ABIN152904) Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immun...
Western Blotting (WB) image for anti-V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog A (Avian) (MAFA) (C-Term) antibody (ABIN152904) Detection of MafA, MafB and cMaf by Western Blot. Samples: Nuclear extract (6 ug) fro...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog A (Avian) (MAFA) (C-Term) antibody (ABIN152904) Immunohistochemistry-Paraffin: MafA Antibody [ABIN152904] - Immunohistochemical stain...
Product cited in: Wei, Yoshihara, He, Hah, Fan, Pinto, Huddy, Wang, Ross, Estepa, Dai, Ding, Sherman, Fang, Zhao, Liddle, Atkins, Yu, Downes, Evans: "Vitamin D Switches BAF Complexes to Protect β Cells." in: Cell, Vol. 173, Issue 5, pp. 1135-1149.e15, 2018 (PubMed).

Blanchet, Van de Velde, Matsumura, Hao, LeLay, Kaestner, Montminy: "Feedback inhibition of CREB signaling promotes beta cell dysfunction in insulin resistance." in: Cell reports, Vol. 10, Issue 7, pp. 1149-57, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Akai, Hosoda, Yoshino, Iwawaki: "Constitutive role of GADD34 and CReP in cancellation of phospho-eIF2α-dependent translational attenuation and insulin biosynthesis in pancreatic β cells." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 20, Issue 11, pp. 871-86, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Chromatin Immunoprecipitation

Alejandro, Gregg, Wallen, Kumusoglu, Meister, Chen, Merrins, Satin, Liu, Arvan, Bernal-Mizrachi: "Maternal diet-induced microRNAs and mTOR underlie β cell dysfunction in offspring." in: The Journal of clinical investigation, Vol. 124, Issue 10, pp. 4395-410, 2014 (PubMed).

Francius, Harris, Rucchin, Hendricks, Stam, Barber, Kurek, Grosveld, Pierani, Goulding, Clotman: "Identification of multiple subsets of ventral interneurons and differential distribution along the rostrocaudal axis of the developing spinal cord." in: PLoS ONE, Vol. 8, Issue 8, pp. e70325, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Hu, Huang, Li, Guo, Cheng: "c-Maf is required for the development of dorsal horn laminae III/IV neurons and mechanoreceptive DRG axon projections." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 16, pp. 5362-73, 2012 (PubMed).

Campbell-Thompson, Heiple, Montgomery, Zhang, Schneider: "Staining protocols for human pancreatic islets." in: Journal of visualized experiments : JoVE, Issue 63, pp. e4068, 2012 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Lausier, Diaz, Roskens, LaRock, Herzer, Fong, Latour, Peshavaria, Jetton: "Vagal control of pancreatic ß-cell proliferation." in: American journal of physiology. Endocrinology and metabolism, Vol. 299, Issue 5, pp. E786-93, 2010 (PubMed).

Artner, Hang, Mazur, Yamamoto, Guo, Lindner, Magnuson, Stein: "MafA and MafB regulate genes critical to beta-cells in a unique temporal manner." in: Diabetes, Vol. 59, Issue 10, pp. 2530-9, 2010 (PubMed).

Guo, Burnette, Zhao, Vanderford, Poitout, Hagman, Henderson, Ozcan, Wadzinski, Stein: "The stability and transactivation potential of the mammalian MafA transcription factor are regulated by serine 65 phosphorylation." in: The Journal of biological chemistry, Vol. 284, Issue 2, pp. 759-65, 2009 (PubMed).

Yang, Chang, Samson, Li, Chan: "The Krüppel-like zinc finger protein Glis3 directly and indirectly activates insulin gene transcription." in: Nucleic acids research, Vol. 37, Issue 8, pp. 2529-38, 2009 (PubMed).

Takeuchi, Kudo, Ogata, Hamada, Nakamura, Kito, Abe, Ueda, Yamamoto, Engel, Takahashi: "Neither MafA/L-Maf nor MafB is essential for lens development in mice." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 14, Issue 8, pp. 941-7, 2009 (PubMed).

Kang, Kim, ZeRuth, Beak, Gerrish, Kilic, Sosa-Pineda, Jensen, Pierreux, Lemaigre, Foley, Jetten: "Transcription factor Glis3, a novel critical player in the regulation of pancreatic beta-cell development and insulin gene expression." in: Molecular and cellular biology, Vol. 29, Issue 24, pp. 6366-79, 2009 (PubMed).

Wang, Torres-Gonzalez, Tripathy, Botolin, Christian, Jump: "Elevated hepatic fatty acid elongase-5 activity affects multiple pathways controlling hepatic lipid and carbohydrate composition." in: Journal of lipid research, Vol. 49, Issue 7, pp. 1538-52, 2008 (PubMed).

Seo, Kim, Lee, Min, Kim, Kim, Won, Park, Lee, Choi, Park, Lee: "Endoplasmic reticulum stress-induced activation of activating transcription factor 6 decreases insulin gene expression via up-regulation of orphan nuclear receptor small heterodimer partner." in: Endocrinology, Vol. 149, Issue 8, pp. 3832-41, 2008 (PubMed).

Wang, Mu, Schraw, Gautron, Elmquist, Zhang, Brownlee, Scherer: "PANIC-ATTAC: a mouse model for inducible and reversible beta-cell ablation." in: Diabetes, Vol. 57, Issue 8, pp. 2137-48, 2008 (PubMed).

Goldsworthy, Hugill, Freeman, Horner, Shimomura, Bogani, Pieles, Mijat, Arkell, Bhattacharya, Ashcroft, Cox: "Role of the transcription factor sox4 in insulin secretion and impaired glucose tolerance." in: Diabetes, Vol. 57, Issue 8, pp. 2234-44, 2008 (PubMed).

Artner, Hang, Guo, Gu, Stein: "MafA is a dedicated activator of the insulin gene in vivo." in: The Journal of endocrinology, Vol. 198, Issue 2, pp. 271-9, 2008 (PubMed).

Vanhoose, Samaras, Artner, Henderson, Hang, Stein: "MafA and MafB regulate Pdx1 transcription through the Area II control region in pancreatic beta cells." in: The Journal of biological chemistry, Vol. 283, Issue 33, pp. 22612-9, 2008 (PubMed).

Zhou, Brown, Kanarek, Rajagopal, Melton: "In vivo reprogramming of adult pancreatic exocrine cells to beta-cells." in: Nature, Vol. 455, Issue 7213, pp. 627-32, 2008 (PubMed).

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