p300 antibody (E1A Binding Protein P300) (AA 1000-1050)

Details for Product anti-EP300 Antibody No. ABIN152981, Supplier: Log in to see
Antigen
  • KAT3B
  • RSTS2
  • p300
  • A430090G16
  • A730011L11
  • p300 HAT
  • E1A binding protein p300
  • histone acetyltransferase p300
  • EP300
  • LOC446056
  • ep300
  • Ep300
Alternatives
anti-Human p300 antibody for Immunoprecipitation
Epitope
AA 1000-1050
56
43
39
19
18
11
6
5
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4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
271
125
112
39
37
3
3
3
3
3
2
Host
Rabbit
178
103
Clonality
Polyclonal
Conjugate
This p300 antibody is un-conjugated
11
11
11
8
8
6
4
4
2
2
2
2
2
2
2
2
2
2
2
2
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2
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2
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
193
111
97
72
56
48
47
38
28
10
7
5
3
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen The immunogen this antibody was made to, maps to a region between residues 1000 and 1050 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).
Purification Immunogen affinity purified
Plasmids, Primers & others Plasmids, Primers & others p300 products on genomics-online (e.g. as negative or positive controls)
Alternative Name KAT3B/p300 (EP300 Antibody Abstract)
Background Gene Symbol: EP300
Gene ID 2033
UniProt Q09472
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size
Application Notes Western Blot 1:1000-1:10000, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:500 to 1:2000, Immunocytochemistry/Immunofluorescence 1:250 - 1:1000, Immunoprecipitation 1-4 μg/mg of lysate, Immunohistochemistry-Paraffin 1:500 to 1:2000Western blot and immunoprecipitation. ChIP reported successful by customer review. Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-E1A Binding Protein P300 (EP300) (AA 1000-1050) antibody (ABIN152981) anti-E1A Binding Protein P300 (EP300) (AA 1000-1050) antibody
Western Blotting (WB) image for anti-E1A Binding Protein P300 (EP300) (AA 1000-1050) antibody (ABIN152981) Western Blot: KAT3B/p300 Antibody [ABIN152981] - Nuclear (NE) or cytosolic (S100) ext...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-E1A Binding Protein P300 (EP300) (AA 1000-1050) antibody (ABIN152981) Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [ABIN152981] - Human lung carcinoma.
Western Blotting (WB) image for anti-E1A Binding Protein P300 (EP300) (AA 1000-1050) antibody (ABIN152981) Western Blot: KAT3B/p300 Antibody - Detection of Human p300 by Western Blot. Samples...
Product cited in: Lee, Huang, Chang, Huang, Hsieh, Chen, Chou, Chiang, Lai, Shiau: "Genome-wide analysis of HIF-2α chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions." in: Scientific reports, Vol. 6, pp. 29311, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Luo, Chen, Chen, Alkam, Wang, Semenza: "PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia." in: Oncotarget, Vol. 7, Issue 6, pp. 6379-97, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Luo, Hu, Chang, Zhong, Knabel, OMeally, Cole, Pandey, Semenza: "Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1." in: Cell, Vol. 145, Issue 5, pp. 732-44, 2011 (PubMed).

Julien, Carriere, Moreau, Roux: "mTORC1-activated S6K1 phosphorylates Rictor on threonine 1135 and regulates mTORC2 signaling." in: Molecular and cellular biology, Vol. 30, Issue 4, pp. 908-21, 2010 (PubMed).

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