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Tumor Necrosis Factor antibody (Tumor Necrosis Factor alpha) Primary Antibody

TNF Reactivity: Human, Mouse, Primate, Rat IHC (p), IHC, ELISA, WB Host: Rabbit Polyclonal
Pubmed (17)
Catalog No. ABIN153474
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  • Target
    Reactivity
    Human, Mouse, Primate, Rat
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This Tumor Necrosis Factor antibody is un-conjugated
    Application
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Specificity
    The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNG beta (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
    Purification
    Delipidation and Defibrination
    Immunogen
    Recombinant full length protein produced in E. coli (Human).
  • Target
    Alternative Name
    TNF-alpha (TNF Antibody Abstract)
    Background
    Gene Symbol: TNF
    Gene ID
    7124
    Pathways
    NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
  • Application Notes
    Western Blot 1:500 - 1:2000, ELISA 1:1000 - 1:5000, Immunohistochemistry 1:100 - 1:500, Immunohistochemistry-Paraffin 1:100 - 1:500
    Comment

    The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    75 mg/mL
    Buffer
    None
    Buffer contains: No Preservative
    Preservative
    Without preservative
    Handling Advice
    Avoid freeze-thaw cycles
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
  • Song, Wu, Yang, Kalueff, Tsao, Dong, Su: "Astrocyte-Conditioned Medium Protects Prefrontal Cortical Neurons from Glutamate-Induced Cell Death by Inhibiting TNF-α Expression." in: Neuroimmunomodulation, Vol. 26, Issue 1, pp. 33-42, 2019 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

    Singh, Devi, Kumawat, Sadam, Appukuttan, Patel, Lingaraju, Singh, Kumar: "Angiogenic and MMPs modulatory effects of icariin improved cutaneous wound healing in rats." in: European journal of pharmacology, Vol. 858, pp. 172466, 2019 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

    Lai, Ho, Evashwick-Rogler, Watanabe, Salandra, Winkelstein, Laudier, Hecht, Pasinetti, Iatridis: "Dietary polyphenols as a safe and novel intervention for modulating pain associated with intervertebral disc degeneration in an in-vivo rat model." in: PLoS ONE, Vol. 14, Issue 10, pp. e0223435, 2019 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

    Bhowmick, DMello, Caruso, Abdul-Muneer: "Traumatic brain injury-induced downregulation of Nrf2 activates inflammatory response and apoptotic cell death." in: Journal of molecular medicine (Berlin, Germany), Vol. 97, Issue 12, pp. 1627-1641, 2019 (PubMed).

    Abdel-Zaher, Farghaly, El-Refaiy, Abd-Eldayem: "Protective effect of the standardized leaf extract of Ginkgo biloba (EGb761) against hypertension-induced renal injury in rats." in: Clinical and experimental hypertension (New York, N.Y. : 1993), Vol. 40, Issue 8, pp. 703-714, 2018 (PubMed).

    Cesur, Gülle, Şirin, Akpolat, Öğrenim, Alkan, Cesur: "Effects of curcumin and melatonin on bone formation in orthopedically expanded suture in rats: A biochemical, histological and immunohistochemical study." in: Orthodontics & craniofacial research, 2018 (PubMed).

    Evashwick-Rogler, Lai, Watanabe, Salandra, Winkelstein, Cho, Hecht, Iatridis: "Inhibiting tumor necrosis factor-alpha at time of induced intervertebral disc injury limits long-term pain and degeneration in a rat model." in: JOR spine, Vol. 1, Issue 2, 2018 (PubMed). (Sample species: Rat (Rattus)).

    Fauzia, Barbhuyan, Shrivastava, Kumar, Garg, Khan, Robertson, Raza: "Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism." in: Frontiers in pharmacology, Vol. 9, pp. 1034, 2018 (PubMed). (Sample species: Chicken).

    Al-Khalaf, Amir, Al-Mohanna, Tulbah, Al-Sayed, Aboussekhra: "Obesity and p16(INK4A) Downregulation Activate Breast Adipocytes and Promote Their Protumorigenicity." in: Molecular and cellular biology, Vol. 37, Issue 17, 2017 (PubMed). (Sample species: Human).

    Rossi, Cadamuro, Biselli-Périco, Leite, Severino, Reis, Cordeiro, Silva: "Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection." in: Cellular microbiology, Vol. 18, Issue 10, pp. 1444-58, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

    Chen, Jian, Lien, Lin, Ting, Chen, Hsu, Huang, Wu, Kuan, Chao, Wu, Huang, Juan: "Adipocytes play an etiological role in the podocytopathy of high-fat diet-fed rats." in: The Journal of endocrinology, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

    Günel, Demirci, Eryılmaz, Yılmaz, Meteoğlu, Ömürlü, Başal: "Inhibitory Effect of Pycnogenol(®) on Airway Inflammation in Ovalbumin-Induced Allergic Rhinitis." in: Balkan medical journal, Vol. 33, Issue 6, pp. 620-626, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Rat (Rattus)).

    Chen, Zhang, Wang, Lei, Su, Zeng, Pei, Huang: "Inhibition of immunoproteasome reduces infarction volume and attenuates inflammatory reaction in a rat model of ischemic stroke." in: Cell death & disease, Vol. 6, pp. e1626, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

    Tessier, Raha, Holloway, Yockell-Lelièvre, Tayade, Gruslin: "Characterization of immune cells and cytokine localization in the rat utero-placental unit mid- to late gestation." in: Journal of reproductive immunology, Vol. 110, pp. 89-101, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

    Wang, Parrish: "Loss of α(E)-catenin promotes Fas mediated apoptosis in tubular epithelial cells." in: Apoptosis : an international journal on programmed cell death, Vol. 20, Issue 7, pp. 921-9, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

    Rodriguez, Edrey, Osmulski, Gaczynska, Buffenstein: "Altered composition of liver proteasome assemblies contributes to enhanced proteasome activity in the exceptionally long-lived naked mole-rat." in: PLoS ONE, Vol. 7, Issue 5, pp. e35890, 2012 (PubMed). Further details: Western Blotting

    Roth, Städler, Lemann, Hösli, Simon, Simon: "Distinct eosinophil cytokine expression patterns in skin diseases - the possible existence of functionally different eosinophil subpopulations." in: Allergy, Vol. 66, Issue 11, pp. 1477-86, 2011 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

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