Myc Tag antibody

Catalog No. ABIN1573879

Product Details anti-Myc Tag Antibody

Target: Myc Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myc Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF)

Sequence: EQKLISEEDL

Specificity: This Antibody recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins.

Purification: Immunoaffinity chromatography

Immunogen: c-Myc epitope tag peptide EQKLISEEDL conjugated - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Immunofluorescence: 5-20 µg/mLImmunoprecipitation (IP): 2-5 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 0.5 mg/ml.

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-Myc Tag antibody (ABIN1573879)

Capella, Ré, Arce, Chan: "Plant homeodomain-leucine zipper I transcription factors exhibit different functional AHA motifs that selectively interact with TBP or/and TFIIB." in: Plant cell reports, Vol. 33, Issue 6, pp. 955-67, (2014) (PubMed).

Armengot, Marquès-Bueno, Soria-Garcia, Müller, Munné-Bosch, Martínez: "Functional interplay between protein kinase CK2 and salicylic acid sustains PIN transcriptional expression and root development." in: The Plant journal : for cell and molecular biology, Vol. 78, Issue 3, pp. 411-23, (2014) (PubMed).

Berim, Park, Gang: "Unexpected roles for ancient proteins: flavone 8-hydroxylase in sweet basil trichomes is a Rieske-type, PAO-family oxygenase." in: The Plant journal : for cell and molecular biology, Vol. 80, Issue 3, pp. 385-95, (2014) (PubMed).

Chen, Sheng, Liu, Yao, Gao, Song, Jiang, Li, Huang: "Enhancer of zeste homolog 2 is a negative regulator of mitochondria-mediated innate immune responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 5, pp. 2614-23, (2013) (PubMed).

Zhang, Xiang, Zhong, Yin, Gu, Deng: "The p16-specific reactivation and inhibition of cell migration through demethylation of CpG islands by engineered transcription factors." in: Human gene therapy, Vol. 23, Issue 10, pp. 1071-81, (2012) (PubMed).

Li, Che, Zou, Cui, Guo, Zou, Biddle, Yang, Chen: "Hpa2 required by HrpF to translocate Xanthomonas oryzae transcriptional activator-like effectors into rice for pathogenicity." in: Applied and environmental microbiology, Vol. 77, Issue 11, pp. 3809-18, (2011) (PubMed).

Xu, Zhang, Schläppi, Xu: "Cold-inducible expression of AZI1 and its function in improvement of freezing tolerance of Arabidopsis thaliana and Saccharomyces cerevisiae." in: Journal of plant physiology, Vol. 168, Issue 13, pp. 1576-87, (2011) (PubMed).

Masuho, Wakasugi-Masuho, Posokhova, Patton, Martemyanov: "Type 5 G protein beta subunit (Gbeta5) controls the interaction of regulator of G protein signaling 9 (RGS9) with membrane anchors." in: The Journal of biological chemistry, Vol. 286, Issue 24, pp. 21806-13, (2011) (PubMed).

Georgakopoulos, Xu, Xu, Mauger, Barthet, Robakis: "Presenilin1/gamma-secretase promotes the EphB2-induced phosphorylation of ephrinB2 by regulating phosphoprotein associated with glycosphingolipid-enriched microdomains/Csk binding protein." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 10, pp. 3594-604, (2011) (PubMed).

Zheng, Li, Zhang, Meng, Mao, Bai, Lu, Mao, Hu, Wang: "Enterovirus 71 2C protein inhibits TNF-?-mediated activation of NF-?B by suppressing I?B kinase ? phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 5, pp. 2202-12, (2011) (PubMed).

Lo, Chen: "CCAAT/enhancer binding protein beta2 is involved in growth hormone-regulated insulin-like growth factor-II gene expression in the liver of rainbow trout (Oncorhynchus mykiss)." in: Endocrinology, Vol. 151, Issue 5, pp. 2128-39, (2010) (PubMed).

Li, Yue, Deng, Ueda, Fukunaga, Khuri, Sun: "Protein phosphatase 2A negatively regulates eukaryotic initiation factor 4E phosphorylation and eIF4F assembly through direct dephosphorylation of Mnk and eIF4E." in: Neoplasia (New York, N.Y.), Vol. 12, Issue 10, pp. 848-55, (2010) (PubMed).

Target Details for Myc Tag

Target: Myc Tag

Alternative Name: C-Myc-Tag (Myc Tag Products)

Target Type: Tag

Background: Rabbit Anti-c-Myc-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide.