Trx Tag antibody
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- Target
- Trx Tag
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- Western Blotting (WB), ELISA
- Specificity
- This antibody reacts with the Thioredoxin fusion protein from E. coli and human. Not yet tested in other species.
- Cross-Reactivity (Details)
- Reactivity to other species is not tested yet.
- Purification
- Protein G affinity column
- Immunogen
- Purified recombinant Trx-tag fusion protein
- Clone
- 4C12H10
- Isotype
- IgG2a
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- Application Notes
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Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/mL
Western blot: 1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 mL WB with 2ml of PBST).Other applications: user-optimized - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4, containing 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- Target
- Trx Tag
- Alternative Name
- Trx-Tag
- Target Type
- Tag
- Background
- Mouse Anti-Trx-tag Monoclonal Antibody reacts with the 109-aa Trx-tag sequence encoded by the PET-32a expression vectors from Novagen. Mouse Anti-Trx-tag Monoclonal Antibody is suitable for detecting fusion proteins that contain a Trx-tag.
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