E Tag antibody

Catalog No. ABIN1573902

Product Details anti-E Tag Antibody

Target: E Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Sequence: GAPVPYPDPL EPR

Specificity: This Antibody recognizes C-terminal, N-terminal, and internal E tagged fusion proteins.

Purification: Immunoaffinity chromatography

Immunogen: E epitope tag peptide GAPVPYPDPLEPR conjugated - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm X 8 cm membrane.Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-E Tag antibody (ABIN1573902)

Du, Zhu, Wu, Fang, Zhou: "Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus." in: International journal of nanomedicine, Vol. 14, pp. 9337-9349, (2020) (PubMed).

Target Details for E Tag

Target: E Tag

Alternative Name: E-tag (E Tag Products)

Target Type: Tag

Background: Rabbit Anti-E-tag Polyclonal Antibody is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide. It is purified by immunoaffinity chromatography.