HAT-Tag antibody

Catalog No. ABIN1573907

Product Details

Target: HAT-Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Sequence: KDHLIHNVHK EFHAHAHNK

Specificity: Rabbit Anti-HAT-tag Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal HAT tagged fusion proteins.

Purification: Immunoaffinity chromatography

Immunogen: HAT epitope tag peptide KDHLIHNVHKEFHAHAHNK conjugated - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 5 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH7.4, containing 0.02 % sodium azid

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References

Chandrasekera, Wan, Gizewski, Auchampach, Lasley: "Adenosine A1 receptors heterodimerize with ?1- and ?2-adrenergic receptors creating novel receptor complexes with altered G protein coupling and signaling." in: Cellular signalling, Vol. 25, Issue 4, pp. 736-42, (2013) (PubMed).

Okada, Yamaura, Suzuki, Itoh, Osada, Takahashi: "Molecular characterization and expression analysis of chitinase from the Pacific oyster Crassostrea gigas." in: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol. 165, Issue 2, pp. 83-9, (2013) (PubMed).

Xiao, Zhou, Sun, Feng, Du, Gao, Ji, Yang, Wang, Han, Langford, Lei: "Apa is a trimeric autotransporter adhesin of Actinobacillus pleuropneumoniae responsible for autoagglutination and host cell adherence." in: Journal of basic microbiology, Vol. 52, Issue 5, pp. 598-607, (2012) (PubMed).

Target Details

Target: HAT-Tag

Target Type: Tag

Background: Rabbit Anti-HAT-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide.