PKC gamma antibody

Catalog No. ABIN1573969

Product Details anti-PKC gamma Antibody

Target: PKC gamma (PRKCG) (Protein Kinase C, gamma (PRKCG))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC gamma antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Rabbit Anti-PKCgamma Polyclonal Antibody detects all endogenous human and mouse PKCgamma protein. No cross-reactivity to other'PKC'soforms is detected.

Cross-Reactivity (Details): Rabbit Anti-PKCγ Polyclonal Antibody detects all endogenous human and mouse PKCγ protein. No cross-reactivity to other PKC isoforms is detected.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide (KLH-coupled) derived from human PKCγ

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may depend on secondary antibody affinity, antigen concentration, detection method sensitivity, temperature, incubation length, and many other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are starting recommendations for this product.Western blot: 0.5-2 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for PKC gamma

Target: PKC gamma (PRKCG) (Protein Kinase C, gamma (PRKCG))

Alternative Name: PKCgamma (PRKCG Products)

Synonyms: PRKCG antibody, PKC-gamma antibody, PKCC antibody, PKCG antibody, SCA14 antibody, PKCgamma antibody, Pkcc antibody, Prkcc antibody, PKC antibody, PKCI antibody, Prkc antibody, RATPKCI antibody, protein kinase C gamma antibody, protein kinase C, gamma antibody, protein kinase C gamma type antibody, PRKCG antibody, Prkcg antibody, LOC484316 antibody

Background: Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. It is involved in diverse cellular signaling pathways. Specifically,'PKC'amily members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the'PKC'amily has a specific expression profile and is believed to play a distinct role in cells'PKC'amily is divided into three groups based on different enzymes' cofactor requirements. They are conventional (c)'PKC'soforms, novel (n)'PKC'soforms, and atypical (a'PKC'soforms respectively. Conventional (c)'PKC'soforms consisting alpha, betaI, betaII and gamma require diacylglycerol (DAG), Ca2+, and phospholipid for activation. Novel (n'PKC'soforms consisting delta, epsilon, eta (also known as PKC-L), Theta and µ(the mouse homolog of human PKCµ known as PKD) require DAG but not Ca2+ for activation. Atypical (a'PKC'soforms, consisting zeta, iota and lambda (the mouse homolog of human'PKC'ota) require neither Ca2+ nor DAG. Other than those listed above, a new'PKC'ember has recently been discovered and is referred to as PKCnu. PKCgamma 'PKC'amma) plays a key role in neuronal signal transduction and regulating intercellular communication.Rabbit Anti-PKCgamma Polyclonal Antibody is developed in rabbit using a synthetic peptide (KLH-coupled) derived from human PKCgamma.

Pathways: WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, G-protein mediated Events, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGF Signaling