Calcineurin A antibody (AA 450-500)

Catalog No. ABIN1574039

Product Details anti-Calcineurin A Antibody

Target: Calcineurin A (CAN)

Binding Specificity: AA 450-500

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calcineurin A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Rabbit Anti-Calcineurin A Polyclonal Antibody detects endogenous levels of calcineurin A protein. Sequence homology predicts that it will also react with rat calcineurin A.

Cross-Reactivity (Details): Rabbit Anti-Calcineurin A Polyclonal Antibody detects endogenous levels of calcineurin A protein. Sequence homology predicts that it will also react with rat calcineurin A.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide (KLH-coupled) within AA 450-500 of human calcineurin A protein

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for Calcineurin A

Target: Calcineurin A (CAN)

Alternative Name: Calcineurin A (CAN Products)

Synonyms: Cna antibody, DDBDRAFT_0185021 antibody, DDBDRAFT_0217855 antibody, DDB_0185021 antibody, DDB_0217855 antibody, PPP3CC antibody, CAN A1/PP2B antibody, CG1455 antibody, CNA1 antibody, Can antibody, Cana1 antibody, CnA antibody, CnnA21EF antibody, D14 antibody, DmCanA1-100B antibody, Dmel\\CG1455 antibody, PP2B antibody, PP2B 21EF antibody, PpD14 antibody, canA antibody, pMY4 antibody, calcineurin A antibody, Calcineurin A antibody, CaM-dependent protein phosphatase, catalytic subunit antibody, protein phosphatase 3 catalytic subunit alpha antibody, Calcineurin A1 antibody, protein phosphatase 3 catalytic subunit beta antibody, Cna antibody, NFIA_077970 antibody, ACLA_011200 antibody, PAS_chr3_0414 antibody, canA antibody, PPP3CA antibody, CanA1 antibody, PPP3CB antibody

Background: Calcineurin is a heterodimer protein that binds to Ca2+. A Ca2+/calmodulin-regulated protein phosphatase, it is composed of a 19 kD calcineurin B (CnB) and a 61 kD calmodulin-binding catalytic subunit, calcineurin A (CnA). Calcineurin A was first detected in skeletal muscle and brain tissues, but it has since been found in all cell types, from yeast to mammalian. Calcineurin A may be responsible for the calmodulin activation property of calcineurin. There is evidence suggesting that alternative splicing could give rise to different forms of calcineurin A.Rabbit Anti-Calcineurin A Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 450-500 of human calcineurin A protein (Swiss Prot: Q08209).