NFASC antibody (N-Term)

Catalog No. ABIN1574089

Product Details anti-NFASC Antibody

Target: NFASC (Neurofascin (NFASC))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFASC antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Rabbit Anti-Neurofascin Polyclonal Antibody detects endogenous levels of rat and mouse Neurofascin protein. It is predicted to react with human neurofascin protein according to sequence homology.

Cross-Reactivity (Details): Rabbit Anti-Neurofascin Polyclonal Antibody detects endogenous levels of rat and mouse Neurofascin protein. It is predicted to react with human neurofascin protein according to sequence homology.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide from amino terminal of human Neurofascin .

Isotype: IgG2a

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL Western blot: 1-2 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for NFASC

Target: NFASC (Neurofascin (NFASC))

Alternative Name: Neurofascin (NFASC Products)

Synonyms: sc:d0205 antibody, NF antibody, NRCAML antibody, AA387016 antibody, D430023G06Rik antibody, mKIAA0756 antibody, neurofascin antibody, neurofascin homolog (chicken) a antibody, NFASC antibody, nfasca antibody, Nfasc antibody

Background: Neurofascin is one member of Li family which involves vertebrate L1, Ng-CAM, and Nr-CAM. Neurofascin protein is a nervous system cell adhesion molecule that contains six Ig domains and multiple fibronectin type III repeats in its extracellular region. Expression level of neurofascin is abundant in the nervous system during early development as well as in adult stage. Phosphorylation of the FIGQY tyrosine of neurofascin abolishes ankyrin binding and increases lateral mobility of neurofascin. Phospho-FIGQY neurofascin associates with doublecortin, which probably plays an important role in neuronal migration.Rabbit Anti-Neurofascin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from amino terminal of human Neurofascin. (Swiss Prot: O94856).

Pathways: Cell-Cell Junction Organization