GFP antibody

Catalog No. ABIN1574093

Product Details anti-GFP Antibody

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Specificity: Rabbit Anti-GFP Polyclonal Antibody reacts with either N-terminal or C-terminal GFP fusion proteins. The antibody also reacts with other variants of GFP, such as CFP, YFP, eGFP and GFPuv.

Cross-Reactivity (Details): Rabbit Anti-GFP Polyclonal Antibody reacts with GFP fusion proteins. The antibody also reacts with other variants of GFP, such as CFP, YFP, eGFP and GFPuv.

Purification: Immunoaffinity chromatography

Immunogen: Purified recombinant full-length GFP protein

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunoprecipitation (IP): 2-10 µg/mg of lysateOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-GFP antibody (ABIN1574093)

Tanaka-Matakatsu, Miller, Du: "The homeodomain of Eyeless regulates cell growth and antagonizes the paired domain-dependent retinal differentiation function." in: Protein & cell, Vol. 6, Issue 1, pp. 68-78, (2015) (PubMed).

Cheng, Wang, Xu, Zhu, Hu, Huang: "Discovery of a novel small secreted protein family with conserved N-terminal IGY motif in Dikarya fungi." in: BMC genomics, Vol. 15, pp. 1151, (2015) (PubMed).

Ni, Wang, Zhang, Pang, Liu, Du: "PKD1 is downregulated in non-small cell lung cancer and mediates the feedback inhibition of mTORC1-S6K1 axis in response to phorbol ester." in: The international journal of biochemistry & cell biology, Vol. 60, pp. 34-42, (2015) (PubMed).

Land, Luo, Levin: "Functional domain analysis of the cell division inhibitor EzrA." in: PLoS ONE, Vol. 9, Issue 7, pp. e102616, (2014) (PubMed).

Chan, Seetharaman, Bagg, Selman, Zhang, Kim, Roy: "EVA-1 functions as an UNC-40 Co-receptor to enhance attraction to the MADD-4 guidance cue in Caenorhabditis elegans." in: PLoS genetics, Vol. 10, Issue 8, pp. e1004521, (2014) (PubMed).

Kwong, Kumai, Perry: "Evidence for a role of tight junctions in regulating sodium permeability in zebrafish (Danio rerio) acclimated to ion-poor water." in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, Vol. 183, Issue 2, pp. 203-13, (2013) (PubMed).

Martínez-Vieyra, Vásquez-Limeta, González-Ramírez, Morales-Lázaro, Mondragón, Mondragón, Ortega, Winder, Cisneros: "A role for ?-dystroglycan in the organization and structure of the nucleus in myoblasts." in: Biochimica et biophysica acta, Vol. 1833, Issue 3, pp. 698-711, (2013) (PubMed).

Wang, Ketcham, Schön, Goodman, Wang, Yates, Freire, Schroer, Zheng: "Nudel/NudE and Lis1 promote dynein and dynactin interaction in the context of spindle morphogenesis." in: Molecular biology of the cell, Vol. 24, Issue 22, pp. 3522-33, (2013) (PubMed).

Kulasekara, Kamischke, Kulasekara, Christen, Wiggins, Miller: "c-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility." in: eLife, Vol. 2, pp. e01402, (2013) (PubMed).

Li, Blissard: "Cellular VPS4 is required for efficient entry and egress of budded virions of Autographa californica multiple nucleopolyhedrovirus." in: Journal of virology, Vol. 86, Issue 1, pp. 459-72, (2011) (PubMed).

Alexander, Selman, Seetharaman, Chan, DSouza, Byrne, Roy: "MADD-2, a homolog of the Opitz syndrome protein MID1, regulates guidance to the midline through UNC-40 in Caenorhabditis elegans." in: Developmental cell, Vol. 18, Issue 6, pp. 961-72, (2010) (PubMed).

Zhang, Xing, Liu: "PUMA promotes Bax translocation by both directly interacting with Bax and by competitive binding to Bcl-X L during UV-induced apoptosis." in: Molecular biology of the cell, Vol. 20, Issue 13, pp. 3077-87, (2009) (PubMed).

Arnold, Zimmerman, Li, Lairmore, Green: "Human T-cell leukemia virus type-1 antisense-encoded gene, Hbz, promotes T-lymphocyte proliferation." in: Blood, Vol. 112, Issue 9, pp. 3788-97, (2008) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Synonyms: green fluorescent protein antibody, gfp antibody

Target Type: Viral Protein

Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. The antibodies raised against full-length GFP should also detect YFP and other variants.Rabbit Anti-GFP Polyclonal Antibody is developed in rabbit using purified recombinant full-length GFP protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.