VDAC1 antibody (AA 150-200)

Catalog No. ABIN1574110

Product Details anti-VDAC1 Antibody

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Binding Specificity: AA 150-200

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VDAC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Rabbit Anti-VDAC1 Polyclonal Antibody detects endogenous levels of human, mouse and rat VDAC1.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide within AA 150-200 of human VDAC1 .

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-VDAC1 antibody (ABIN1574110)

Sayed, Yang, He, Pfleger, Abdellatif: "Acute targeting of general transcription factor IIB restricts cardiac hypertrophy via selective inhibition of gene transcription." in: Circulation. Heart failure, Vol. 8, Issue 1, pp. 138-48, (2015) (PubMed).

Sayed, He, Yang, Lin, Abdellatif: "Transcriptional regulation patterns revealed by high resolution chromatin immunoprecipitation during cardiac hypertrophy." in: The Journal of biological chemistry, Vol. 288, Issue 4, pp. 2546-58, (2013) (PubMed).

Target Details for VDAC1

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Alternative Name: VDAC1 (VDAC1 Products)

Synonyms: vdac1 antibody, VDAC1 antibody, 5076 antibody, ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 1 antibody, ATVDAC1 antibody, T22N4.9 antibody, T22N4_9 antibody, voltage dependent anion channel 1 antibody, PORIN antibody, VDAC-1 antibody, AL033343 antibody, Vdac5 antibody, fa13f11 antibody, wu:fa13f11 antibody, zgc:85830 antibody, voltage-dependent anion channel 1 S homeolog antibody, voltage-dependent anion channel 1 antibody, voltage dependent anion channel 1 antibody, vdac1.S antibody, vdac1 antibody, VDAC1 antibody, Vdac1 antibody

Background: Voltage dependent anion selective channel protein 1 (also known as VDAC1 or mitochondrial Porin) is the predominant protein in the outer mitochondrial membrane. VDAC1 is predicted to have a beta-barrel structure and consist of either 13 or 16 membrane spanning strands. In particular, VDAC1 can function as an NADH ferricyanide reductase. It plays a vital role in modulating endostatin induced endothelial cell apoptosis. Its activity is regulated by Nek1 (NIMA related protein kinase 1) through phosphorylation of VDAC1 protein on serine 193.Rabbit Anti-VDAC1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 150-200 of human VDAC1 (Swiss Prot: P21796).