DARPP32 antibody (AA 150-200)

Catalog No. ABIN1574114

Product Details anti-DARPP32 Antibody

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Binding Specificity: AA 150-200

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARPP32 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: Rabbit Anti-DARPP-32 Polyclonal Antibody detects endogenous levels of mouse and rat DARPP-32. Predicted to react with human DARPP-32 according to sequence homology.Positive Control: Mouse brain and rat brain

Cross-Reactivity (Details): Rabbit Anti-DARPP-32 Polyclonal Antibody detects endogenous levels of mouse and rat DARPP-32. Predicted to react with human DARPP-32 according to sequence homology.Positive Control: Mouse brain and rat brain

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide within AA 150-200 of human DARPP-32 .

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Alternative Name: DARPP-32 (PPP1R1B Products)

Background: DARPP-32 (Dopamine- and cAMP-Regulated Neuronal Phosphoprotein, Mr 32 kDa), also known as PPP1R1B, is expressed in medium-sized spiny neurons that also express dopamine D1 receptors. It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine stimulates phosphorylation of DARPP-32 through dopamine D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1. DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5).Rabbit Anti-DARPP-32 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 150-200 of human DARPP-32 (Swiss Prot: Q9UD71).