Lamin A/C antibody (AA 400-450)

Catalog No. ABIN1574125

Product Details anti-Lamin A/C Antibody

Target: Lamin A/C (LMNA)

Binding Specificity: AA 400-450

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin A/C antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: Rabbit Anti-Lamin A+C Polyclonal Antibody detects endogenous levels of human and mouse Lamin A and Lamin C. It is predicted to react with rat Lamin A and Lamin C according to sequence homology.Positive Control: Hela, A431, Lovo and NIH/3T3

Cross-Reactivity (Details): Rabbit Anti-Lamin A+C Polyclonal Antibody detects endogenous levels of human and mouse Lamin A and Lamin C. It is predicted to react with rat Lamin A and Lamin C according to sequence homology.Positive Control: Hela, A431, Lovo and NIH/3T3

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide within AA 400-450 of human Lamin A .

Isotype: IgG1

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: Rabbit Anti-Lamin A+C Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin A+C (LMNA Products)

Synonyms: CDCD1 antibody, CDDC antibody, CMD1A antibody, CMT2B1 antibody, EMD2 antibody, FPL antibody, FPLD antibody, FPLD2 antibody, HGPS antibody, IDC antibody, LDP1 antibody, LFP antibody, LGMD1B antibody, LMN1 antibody, LMNC antibody, LMNL1 antibody, PRO1 antibody, Dhe antibody, 2459 antibody, 74/76 antibody, CG6944 antibody, D5 antibody, DM[[O]] antibody, Dm antibody, Dm(0) antibody, Dm0 antibody, Dm1 antibody, Dm2 antibody, DmLamin antibody, DmO antibody, Dm[[0]] antibody, Dm[[1]] antibody, Dm[[2]] antibody, Dm[[mit]] antibody, Dm[[o]] antibody, Dmel\\CG6944 antibody, Dmo antibody, LAM antibody, Lam Dm[[0]] antibody, Lam(Dm0) antibody, LamDm[[0]] antibody, LamDm[[o]] antibody, Lam[[Dm0]] antibody, Lamin Dm[[0]] antibody, jf27 antibody, l(2)04643 antibody, l(2)25Ec antibody, l(2)gdh-7 antibody, l(2)gdh7 antibody, l(2)jf27 antibody, lam antibody, lamDm0 antibody, lamDm[[0]] antibody, lamin antibody, lamin Dm0 antibody, lamin Dm[[0]] antibody, misg antibody, nlam antibody, cb948 antibody, fk66d12 antibody, wu:fk66d12 antibody, lmna-A antibody, fpl antibody, idc antibody, lfp antibody, cddc antibody, emd2 antibody, fpld antibody, hgps antibody, ldp1 antibody, lmn1 antibody, lmnc antibody, pro1 antibody, cdcd1 antibody, cmd1a antibody, cmt2b1 antibody, lgmd1b antibody, Lamin-A antibody, lamin-L(III)-like antibody, lamin A/C antibody, lamin A antibody, Lamin antibody, lamin A/C L homeolog antibody, lamin-L(III)-like antibody, LMNA antibody, Lmna antibody, Lam antibody, lmna antibody, lmna.L antibody, LMINA antibody

Background: Lamins are structural protein components of the nuclear lamina which contains 3 members: Lamin A, B and C in mammalian cells. Lamin A and lamin C are generated by alternative splicing from the same gene and share complete identity for the first 566 amino acids. Lamin A interacts with transcription factor SREBP1 via its C-terminal domain. The lamin A/C deficiency is probably associated with both defective nuclear mechanics and impaired transcriptional activation. The lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. Rabbit Anti-Lamin A+C Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 400-450 of human Lamin A (Swiss Prot: P02545).

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus