EEA1 antibody (N-Term)

Catalog No. ABIN1574158

Product Details anti-EEA1 Antibody

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Please inquire

Specificity: Rabbit Anti-EEA1 Polyclonal Antibody detects endogenous levels of human, mouse and rat EEA1 protein.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: Rabbit Anti-EEA1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human EEA1 (Swiss Prot: Q15075).

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide from N-terminal of human EEA1

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: Lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

References for anti-EEA1 antibody (ABIN1574158)

Tian, Zhu, Hu, Wang, Huang, Xiao: "Dynamics of exosome internalization and trafficking." in: Journal of cellular physiology, Vol. 228, Issue 7, pp. 1487-95, (2013) (PubMed).

Hazeki, Kametani, Murakami, Uehara, Ishikawa, Nigorikawa, Takasuga, Sasaki, Seya, Matsumoto, Hazeki: "Phosphoinositide 3-kinase? controls the intracellular localization of CpG to limit DNA-PKcs-dependent IL-10 production in macrophages." in: PLoS ONE, Vol. 6, Issue 10, pp. e26836, (2011) (PubMed).

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: EEA1 (EEA1 Products)

Synonyms: MST105 antibody, MSTP105 antibody, ZFYVE2 antibody, im:7151048 antibody, si:ch211-276p10.2 antibody, A430109M19Rik antibody, B230358H09Rik antibody, early endosome antigen 1 antibody, early endosome antigen 1 S homeolog antibody, EEA1 antibody, eea1 antibody, Eea1 antibody, eea1.S antibody

Background: Early endosomal antigen 1 (EEA1) is an early endosomal marker which is essential for fusion between early endocytic vesicles. It interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion. EEA1 protein contains an N-terminal zinc finger motif and a cys-rich C-terminal metal-binding finger. A FYVE domain located at the carboxy terminus of EEA1 binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes.

Molecular Weight: Predicted Band Size: 162 KD
Observed Band Size: 162 KD

UniProt: Q15075

Pathways: Toll-Like Receptors Cascades