INA antibody

Catalog No. ABIN1580440

Product Details anti-INA Antibody

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reactivity: Human, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This INA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: conc. tissue culture supernatant

Clone: 2E3

Isotype: IgG1

Application Details

Application Notes: For immunofluorescence try dilutions of 1:500, for immunohistochemistry with ABC or other enzymatic amplification procedures try 1:5,000. For western blots try 1:10,000 dilutions. On Western blots the antibody is unusually strong and specific in bindingas shown above. The antibody is clean and specific on immunoblots. Can be used on formalin-fixed cells in tissue culture, cryostat sections, and Western blotting. The epitope recognized by the 2E3 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

Target Details for INA

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Abstract: INA Products

Synonyms: NEF5 antibody, NF-66 antibody, TXBP-1 antibody, Inexa antibody, Intlaa antibody, Nf66 antibody, AV028420 antibody, NF66 antibody, ina-A antibody, xefiltin antibody, ina-a antibody, inexa antibody, MGC82496 antibody, INA antibody, si:ch211-67n3.6 antibody, nef5 antibody, xina antibody, nf-66 antibody, txbp-1 antibody, MGC69277 antibody, gefiltin antibody, zgc:110516 antibody, internexin neuronal intermediate filament protein alpha antibody, internexin neuronal intermediate filament protein, alpha antibody, internexin neuronal intermediate filament protein alpha S homeolog antibody, low molecular weight neuronal intermediate filament S homeolog antibody, internexin neuronal intermediate filament protein, alpha a antibody, internexin neuronal intermediate filament protein, alpha b antibody, INA antibody, Ina antibody, ina.S antibody, nif.S antibody, inaa antibody, ina antibody, inab antibody

Background: A-internexin is a Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits. On SDS-PAGE gels it runs with an apparent molecular weight of 64 to 66 kDa, with some species variability, although the real molecular weight is about 55 kDa, as with the other neurofilament subunits the presence of highly negatively charged sequences results in reduction of SDS-PAGE mobility. a-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development procedes. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up-regulation of a-internexin during neuronal regeneration. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Trojanowski, Lee and coworkers used this antibody to show that a-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID, see two Cairns et al. papers listed below), a serious human neurodegenerative disorder. The antibody was also used to confirm the presence of circulating auto-antibodies to a-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (Rajasalu et al., 2004, listed below). The HGNC name for this protein is INA.