DDX60 antibody

Catalog No. ABIN1587945

Product Details anti-DDX60 Antibody

Target: DDX60 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 60 (DDX60))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX60 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: At least three isoforms of DDX60 are known to exist, this antibody will detect all three isoforms.

Purification: Affinity chromatography purified via peptide column

Immunogen: DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60.

Isotype: IgG

Application Details

Application Notes: DDX60 antibody can be used for detection of DDX60 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Comment:

(IN) DEAD box protein 60

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: 4 °C

Storage Comment: DDX60 antibody can be stored at 4 °C for three months. At -20 °C stable for up to one year.

Expiry Date: 12 months

Target Details for DDX60

Target: DDX60 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 60 (DDX60))

Alternative Name: DDX60 (DDX60 Products)

Synonyms: 9830118M07 antibody, RGD1560014 antibody, DExD/H-box helicase 60 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 antibody, DEXD/H-box helicase 60 antibody, DDX60 antibody, Ddx60 antibody

Background: DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.

Molecular Weight: 188 kDa

Gene ID: 55601

NCBI Accession: NP_060101