Fibrinogen antibody (Biotin)

Catalog No. ABIN1607652

Product Details anti-Fibrinogen Antibody (Biotin)

Target: Fibrinogen

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Fibrinogen antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity against Fibrinogen from other sources is unknown.

Purity: Anti-Fibrinogen antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Fibrinogen [Human Plasma].

Endotoxin Level: Low Endotoxin : No

Immunogen: Fibrinogen [Human Plasma]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Fibrinogen antibody has been assayed against 1.0 µg of Fibrinogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:50.000 to 1:250.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:20.000 - 1:100.000
Fluorochrome Protein Value: 10-20
Immunohistochemistry Dilution: 1:1.000 - 1:5.000
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Fibrinogen

Target: Fibrinogen

Abstract: Fibrinogen Products

Synonyms: fib2 antibody, MGC53620 antibody, fibrinogen antibody, sb:cb892 antibody, wu:fa55c12 antibody, wu:fb20e08 antibody, wu:fb59b03 antibody, wu:fi39d04 antibody, zgc:92117 antibody, fibrinogen alpha chain antibody, fibrinogen gamma chain antibody, fga antibody, FGA antibody, LOC698244 antibody, CpipJ_CPIJ010090 antibody, FGG antibody

Background: Anti-Fibrinogen antibody has a dual function: yielding monomers that polymerize into fibrin and as a main protein of the blood coagulation system, it also functions as a cofactor in platelet aggregation. Fibrinogen clotting underlies pathogenesis of thromboembolism, MI, and thrombus formation. Fibrinogen activation is a major factor in the mechanism which causes inflammation, tumor growth and various additional diseases. These characteristics make Anti-Fibrinogen ideal for investigators interested in the fields of Cardiovascular or Metabolism research. Synonyms: FGA antibody, FGA protein antibody, FGB antibody, FGG antibody, Fib2 antibody, Fibrinogen A alpha polypeptide antibody, Fibrinogen A alpha polypeptide chain antibody, Fibrinogen alpha chain antibody

Gene ID: 2243

UniProt: P02671