Papain antibody (Biotin)

Catalog No. ABIN1607673

Product Details

Target: Papain

Reactivity: Papaya

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Papain from other sources may occur but have not been specifically determined.

Purity: Anti-Papain antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Papain [Carica papaya].

Endotoxin Level: Low Endotoxin : No

Immunogen: Papain [Carica papaya]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Papain antibody is suitable for ELISA and Western Blot. Expect band at 38.9 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:2.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:2.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details

Target: Papain

Synonyms: PPI antibody, Papain antibody, papain-like antibody, LOC110813108 antibody

Background: Anti-Papain antibody recognizes the cysteine protease papai commonly found in papaya (Carica papaya). Papain hydrolyzes a broad range of peptides with preference for bulky hydrophobic amino acids at the P2 position. Papain is a single-chained polypeptide stabilized by three disulfide bridges. Its active site contains a catalytic diad composed of cysteine-25 and histidine-159. Anti-Papain antibody is ideal for researchers interested in Cell Biology and Proteolysis. Synonyms: Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody

UniProt: P00784