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AKT3 antibody (Internal Region)

AKT3 Reactivity: Human WB, ELISA, IHC Host: Mouse Monoclonal 9A5-H9-G7 unconjugated
Catalog No. ABIN1607690
  • Target See all AKT3 Antibodies
    AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
    Binding Specificity
    • 14
    • 11
    • 10
    • 8
    • 7
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 108
    • 59
    • 43
    • 11
    • 10
    • 9
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 92
    • 25
    • 1
    Mouse
    Clonality
    • 83
    • 35
    Monoclonal
    Conjugate
    • 57
    • 10
    • 8
    • 8
    • 7
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This AKT3 antibody is un-conjugated
    Application
    • 95
    • 58
    • 40
    • 13
    • 9
    • 7
    • 6
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Cross-Reactivity (Details)
    Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross reactivity with AKT2 and AKT has not been determined.
    Purity
    Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography.
    Endotoxin Level
    Low Endotoxin : No
    Immunogen
    Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.
    Immunogen Type: Peptide
    Clone
    9A5-H9-G7
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product AKT3 Primary Antibody
  • Application Notes
    Anti-AKT3 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
    ELISA Dilution: 1:2.000 - 1:10.000
    Immunohistochemistry Dilution: 20 µg/mL
    Western Blot Dilution: 1:500-1:2000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Handling Advice
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Expiry Date
    Expiration date is one (1) year from date of opening.
  • Target
    AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
    Alternative Name
    AKT3 (AKT3 Products)
    Synonyms
    MPPH antibody, PKB-GAMMA antibody, PKBG antibody, PRKBG antibody, RAC-PK-gamma antibody, RAC-gamma antibody, STK-2 antibody, AI851531 antibody, D930002M15Rik antibody, Nmf350 antibody, Pkbg antibody, AKT serine/threonine kinase 3 antibody, thymoma viral proto-oncogene 3 antibody, v-akt murine thymoma viral oncogene homolog 3b antibody, AKT3 antibody, Akt3 antibody, akt3b antibody
    Background
    AKT3 Antibody detects AKT3 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT3 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research. Synonyms: AKT 3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma
    Gene ID
    10000
    UniProt
    Q9Y243
    Pathways
    PI3K-Akt Signaling, RTK Signaling, TLR Signaling, Hepatitis C, VEGF Signaling
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