DLD antibody

Catalog No. ABIN1607742

Product Details anti-DLD Antibody

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Lipoamide Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Purity: Anti-LIPOAMIDE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart].

Endotoxin Level: Low Endotoxin : No

Immunogen: Lipoamide Dehydrogenase [Porcine Heart]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-LIPOAMIDE DEHYDROGENASE has been assayed against 1.0 µg of Lipoamide Dehydrogenase [Porcine Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:7.000 to 1:30.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for DLD

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Alternative Name: Lipoamide Dehydrogenase (DLD Products)

Synonyms: DLDD antibody, DLDH antibody, E3 antibody, GCSL antibody, LAD antibody, PHE3 antibody, AI315664 antibody, AI746344 antibody, wu:fb24b05 antibody, DLD antibody, DDBDRAFT_0183800 antibody, DDBDRAFT_0216232 antibody, DDB_0183800 antibody, DDB_0216232 antibody, sc:d0402 antibody, dihydrolipoamide dehydrogenase antibody, dihydrolipoyl dehydrogenase antibody, deltaD antibody, DLD antibody, Dld antibody, dldh antibody, AT4G16155 antibody, CND05840 antibody, bfmBC antibody, GCSL antibody, LACBIDRAFT_182385 antibody, UREG_06178 antibody, lpd antibody, TAGG_RS02070 antibody, Arnit_2606 antibody, Mesil_1945 antibody, Trad_2118 antibody, Acear_0640 antibody, Fbal_0372 antibody, Ilyop_1890 antibody, Ftrac_1733 antibody, Ocepr_1753 antibody, Intca_2017 antibody, Deima_0504 antibody, dld antibody

Background: Synonyms: Dihydrolipoyl dehydrogenase, mitochondrial EC=1.8.1.4 Dihydrolipoamide dehydrogenase Lipoamide Dehydrogenase [Porcine Heart]

Gene ID: 397129, 118675

UniProt: P09623

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis