IL-22 antibody (Biotin)

Catalog No. ABIN1607868

Product Details anti-IL-22 Antibody (Biotin)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-22 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity with IL-22 from other sources has not been determined.

Purity: Anti-IL-22 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-22 protein. A BLAST analysis was used to suggest cross-reactivity with IL-22 from human sources based on 100 % homology with the immunizing sequence. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum.

Endotoxin Level: Low Endotoxin : No

Immunogen: Anti-IL-22 antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human IL-22 protein from E. coli.
Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes: IL-22 purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 20 kDa in size corresponding to the human IL-22 protein by western blotting in appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:50.000
Fluorochrome Protein Value: 10-20
Western Blot Dilution: 1:1000-1:2000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with 100 µL deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for IL-22

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: IL-22 (IL22 Products)

Synonyms: IL-21 antibody, IL-22 antibody, IL-D110 antibody, IL-TIF antibody, ILTIF antibody, TIFIL-23 antibody, TIFa antibody, zcyto18 antibody, IL-22a antibody, ILTIFa antibody, Iltif antibody, RGD1561292 antibody, interleukin-22 antibody, interleukin 22 antibody, IL22 antibody, il22 antibody, Il22 antibody

Background: Interleukin-22 (IL-22) is a member of a group of cytokines called IL-10 family member, produced by activated dendritic cells and T lymphocytes. IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the liver and pancreas. IL-22 signals via the IL-22R and IL-10Rß receptors to act as a potent mediator of the cellular inflammatory response. Recombinant human IL-22 is a non-disulfide-linked homodimer with a total molecular weight of 33.6 kDa. Anti-IL-22 antibody is ideal for researchers interested in cytokines and stem cell research. Synonyms: Interleukin-22, ILTIF, ZCYTO18, IL22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor

Gene ID: 50616

NCBI Accession: NP_065386

UniProt: Q9GZX6