KMO antibody (Internal Region)

Catalog No. ABIN1607892

Product Details anti-KMO Antibody

Target: KMO (Kynurenine 3-Monooxygenase (Kynurenine 3-Hydroxylase) (KMO))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KMO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity (Details): Cross reactivity with KMO from other sources has not been determined.

Purity: Anti-KMO was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards Kynurenine 3-monooxygenase (KMO). A BLAST analysis was used to suggest cross-reactivity with Mouse based on 100 % sequence homology.

Endotoxin Level: Low Endotoxin : No

Immunogen: KMO affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the mouse KMO chain.
Immunogen Type: Peptide

Application Details

Application Notes: Anti-KMO antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~58 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:60.000
Immunohistochemistry Dilution: 1:100-1:500
IF Microscopy Dilution: 1:100-1:500
Western Blot Dilution: 1 µg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.08 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: Expiration date is six (6) months from date of opening.

Target Details for KMO

Target: KMO (Kynurenine 3-Monooxygenase (Kynurenine 3-Hydroxylase) (KMO))

Alternative Name: KMO (KMO Products)

Synonyms: AI046660 antibody, dJ317G22.1 antibody, kynurenine 3-monooxygenase (kynurenine 3-hydroxylase) antibody, kynurenine 3-monooxygenase antibody, Kmo antibody, KMO antibody

Background: Kynurenine 3-monooxygenase (KMO) catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). The enzyme is required for synthesis of quinolinic acid. Quinolinic acid is a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Anti-KMO antibodies are ideal for researchers interested in Apoptosis, Neurodegeneration, and Neuroscience research. Synonyms: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)

Gene ID: 8564

NCBI Accession: NP_003670

UniProt: O15229