CD8B antibody (FITC)

Catalog No. ABIN161260

Product Details anti-CD8B Antibody (FITC)

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8B antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: Rat CD8b

Immunogen: Rat/mouse T cell hybrids expressing CD8 . Fusion Partner: X63 Ag.653 myeloma cell line . Donor: BALB/c mouse spleen cells

Clone: 3-4-1

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.1 mg/ml

Buffer: FITC conjugated Ig buffered in PBS, 0.02% NaN and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography).

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8b (CD8B Products)

Synonyms: Cd8b antibody, Ly-3 antibody, Ly-C antibody, Lyt-3 antibody, CD8B1 antibody, LEU2 antibody, LY3 antibody, LYT3 antibody, P37 antibody, Cd8b1 antibody, CD8b molecule antibody, CD8 antigen, beta chain 1 antibody, Cd8b antibody, CD8B antibody, Cd8b1 antibody

Background: Anti-rat CD8b monoclonal antibody reacts with the beta chain of the CD8 differentiation antigen. CD8b is expressed on most thymocytes and mature T cytotoxic/ suppressor cells (MHC class I restricted). While the CD8a and CD8b form a heterodimer on the surface of thymocytes and thymus-dependent T cytotoxic/suppressor cells, the majority of NK cells, many CD8 T cells from athymic rats, many activated CD4 T cells, and intestinal epithelium lymphocytes (IEL) express CD8a without CD8b. This suggests that expression of the CD8 heterodimer (a/b) is more dependant on intrathymic T cell maturation than that of the homodimer (a/a). The thymus dependence of CD8a/b T cells may be due to a requirement for thymic selection on self MHC class I antigens. Reported applications for this antibody include flow cytometry, immunoprecipitation and Western blotting. The 3.4.1 antibody also blocks both activation in an allogenic response and cell mediated cytotoxicity by CD8 T cells.

Pathways: TCR Signaling