ATP2A2 antibody (Internal Region)
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- Target See all ATP2A2 Antibodies
- ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This ATP2A2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purpose
- SERCA2 / ATP2A2
- Sequence
- DELNPSAQRD ACLN
- Specificity
- This antibody is expected to recognize both reported isoforms (NP_001672.1, NP_733765.1).
- Cross-Reactivity
- Dog, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
- Top Product
- Discover our top product ATP2A2 Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.
Western Blot: Approx 110 kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 110 kDa according to NP_001672.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:128000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum of HeLa cells. Recommended concentration: 10μg/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))
- Alternative Name
- ATP2A2 (ATP2A2 Products)
- Synonyms
- atp2b antibody, ca-p60a antibody, dar antibody, serca2 antibody, ATP2A2 antibody, ATP2B antibody, DAR antibody, DD antibody, SERCA2 antibody, SERCA2A antibody, ATP2 antibody, Serca2 antibody, SercaII antibody, 9530097L16Rik antibody, D5Wsu150e antibody, SERCA2B antibody, mKIAA4195 antibody, atp2a2 antibody, zgc:55380 antibody, ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 S homeolog antibody, ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 antibody, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 antibody, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a antibody, atp2a1.S antibody, ATP2A2 antibody, Atp2a2 antibody, atp2a2a antibody
- Background
- ATP2A2, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2, ATP2B, DAR, DD, DKFZp686P0211, FLJ20293, FLJ38063, MGC45367, SERCA2, ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2, SR Ca(2+)-ATPase 2, calcium pump 2, calcium-transporting ATPase s
- Molecular Weight
- 110 kDa
- Gene ID
- 488, 11938, 29693
- NCBI Accession
- NP_001672, NP_733765
- Pathways
- Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process
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