TRAIL antibody (AA 95-281) (PE)

Catalog No. ABIN1690743

Product Details anti-TRAIL Antibody (PE)

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Binding Specificity: AA 95-281

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRAIL antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The antibody 2E5 reacts with an extracellular epitope within C-terminal half of TRAIL (APO-2L), a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate and also in many other tissues.

No Cross-Reactivity: Mouse

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant soluble fragment (aa 95-281) of human TRAIL.

Clone: 2E5

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-TRAIL antibody (PE) (ABIN1690743)

Hyer, Croxton, Krajewska, Krajewski, Kress, Lu, Suh, Sporn, Cryns, Zapata, Reed: "Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells." in: Cancer research, Vol. 65, Issue 11, pp. 4799-808, (2005) (PubMed).

Plasilova, Zivny, Jelinek, Neuwirtova, Cermak, Necas, Andera, Stopka: "TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors." in: Leukemia, Vol. 16, Issue 1, pp. 67-73, (2002) (PubMed).

Target Details for TRAIL

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Alternative Name: CD253 / TRAIL (TNFSF10 Products)

Synonyms: APO2L antibody, Apo-2L antibody, CD253 antibody, TL2 antibody, TRAIL antibody, A330042I21Rik antibody, AI448571 antibody, APO-2L antibody, Ly81 antibody, Trail antibody, tl2 antibody, apo2l antibody, cd253 antibody, trail antibody, apo-2l antibody, trail1 antibody, xtrail1 antibody, tnfsf10l2 antibody, zgc:92320 antibody, TNLG6A antibody, TNF superfamily member 10 antibody, tumor necrosis factor (ligand) superfamily, member 10 antibody, tumor necrosis factor superfamily member 10 antibody, TNF superfamily member 10 S homeolog antibody, TNFSF10 antibody, Tnfsf10 antibody, tnfsf10.S antibody, tnfsf10 antibody

Background: TNF superfamily member 10,Human CD253 / TRAIL (TNF-related apoptosis inducing ligand), also called Apo2, is a type II membrane protein from the TNF family. TRAIL is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apotosis, is achieved through binding to two dealth-signaling receptors, DR4 (CD261 / TRAIL-R1) and DR5 (CD262 / TRAIL-R2).,TRAIL, Apo-2 ligand, Apo-2L, TNFSF10, APO2L, TNLG6A

Gene ID: 8743

UniProt: P50591

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity