GGA1 antibody (AA 51-150) (Cy3)

Catalog No. ABIN1703111

Product Details anti-GGA1 Antibody (Cy3)

Target: GGA1 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1))

Binding Specificity: AA 51-150

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GGA1 antibody is conjugated to Cy3

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GGA1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GGA1

Target: GGA1 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1))

Alternative Name: GGA1 (GGA1 Products)

Synonyms: 4930406E12Rik antibody, AU016030 antibody, AW209092 antibody, fb33h10 antibody, im:6912200 antibody, wu:fb33h10 antibody, zgc:101044 antibody, putative golgi associated, gamma adaptin ear containing,arf binding protein 1 (gga1) antibody, golgi associated, gamma adaptin ear containing, ARF binding protein 1 antibody, golgi-associated, gamma adaptin ear containing, ARF binding protein 1 antibody, Smp_164030 antibody, Gga1 antibody, gga1 antibody, GGA1 antibody

Background:

Synonyms: 4930406E12Rik, ADP ribosylation factor binding protein 1, ADP ribosylation factor binding protein GGA1, ADP-ribosylation factor-binding protein GGA1, ARF binding protein 1, ARF-binding protein 1, Gamma adaptin related protein 1, gamma ear-containing, Gamma-adaptin-related protein 1, GGA 1, GGA1, GGA1 protein, GGA1_HUMAN, Golgi associated gamma adaptin ear containing ARF, Golgi associated gamma adaptin ear containing ARF binding protein 1, Golgi localized gamma ear containing ARF binding protein 1, Golgi-localized, OTTHUMP00000028975, OTTHUMP00000042200.

Background: The GGA family of proteins (Golgi-localized, ARF-binding proteins) are ubiquitous coat proteins that facilitate the trafficking of soluble proteins from the trans-Golgi network (TGN) to endosomes/lysosomes by means of interactions with TGN-sorting receptors, ARF (ADP-ribosylation factor), and clathrin (1?). Members of the GGA family, GGA1,GGA2 (also known as VEAR) and GGA3, are multidomain proteins that bind mannose 6-phosphate receptors (MPRs) (1,2,4). GGAs have modular structures with an N-terminal VHS (VPS-27, Hrs, and STAM) domain followed by a GAT (GGA and TOM1) domain, a connecting hinge segment, and a C-terminal GAE (?adaptin ear) domain (5). The amino-terminal VHS domains of GGAs form complexes with the cytoplasmic domains of sorting receptors by recognizing acidic-cluster di-leucine (ACLL) sequences (3). GGA1 and GGA2 do not associate with each other, but they do colocalize on perinuclear membranes (2). The cytosolic domain of memapsin 2, but not that of memapsin 1, binds the VHS domains of GGA1 and GGA2 (6). The human GGA1 gene maps to chromosome 22 and encodes a protein that shares 45 % sequence identity with GGA2 and GGA3 (1).

Gene ID: 26088