CSTF3 antibody (AA 151-250) (Cy5)

Catalog No. ABIN1704168

Product Details anti-CSTF3 Antibody (Cy5)

Target: CSTF3 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3))

Binding Specificity: AA 151-250

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSTF3 antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CSTF3/CSTF77

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CSTF3

Target: CSTF3 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3))

Alternative Name: CSTF3/CSTF77 (CSTF3 Products)

Synonyms: cstf3 antibody, MGC76035 antibody, CSTF3 antibody, wu:fa99c02 antibody, wu:fb18b09 antibody, zgc:56028 antibody, 4732468G05Rik antibody, C79532 antibody, CstF-77 antibody, cstf3a antibody, CSTF-77 antibody, cleavage stimulation factor, 3' pre-RNA, subunit 3 antibody, cleavage stimulation factor subunit 3 antibody, cstf3 antibody, CSTF3 antibody, LOC100544392 antibody, LOC100641283 antibody, Cstf3 antibody, cstf3.S antibody

Background:

Synonyms: CF 1 77 kDa subunit, Cleavage stimulation factor 77 kDa subunit, Cleavage stimulation factor subunit 3, Cleavage stimulation factor subunit 3 isoform 1, CSTF 3, CSTF 77, CSTF 77 kDa subunit, CSTF77, MGC117398, MGC43001, MGC75122, CSTF3_HUMAN.

Background: Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA. CstF-77 (cleavage stimulation factor, 77 kDa subunit), also known as CstF3, is one of the three subunits comprising CstF. It can exist as a homodimer and functions as the bridge, directly interacting with the other two CstF subunits, namely CstF-64 and CstF-50. CstF-77 is highly conserved among eukaryotes. It contains an Alpha-helical structure with 11 HAT (Half-a-TPR-containing) repeats and is essential for CstF assembly. In addition, CstF-77 is capable of interacting with CPSF1 and FCP1, other factors involved in polyadenylation.

Gene ID: 1479