Destrin antibody (AA 75-165) (HRP)

Catalog No. ABIN1712033

Product Details anti-Destrin Antibody (HRP)

Target: Destrin (DSTN) (Destrin (Actin Depolymerizing Factor) (DSTN))

Binding Specificity: AA 75-165

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Destrin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Horse,Rabbit,Monkey

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Destrin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Destrin

Target: Destrin (DSTN) (Destrin (Actin Depolymerizing Factor) (DSTN))

Alternative Name: Destrin (DSTN Products)

Synonyms: ACTDP antibody, ADF antibody, bA462D18.2 antibody, DSTN antibody, LOC100190417 antibody, adf antibody, actdp antibody, ba462d18.2 antibody, 2610043P17Rik antibody, AU042046 antibody, Dsn antibody, corn1 antibody, sid23p antibody, destrin antibody, destrin, actin depolymerizing factor antibody, destrin antibody, destrin, actin depolymerizing factor S homeolog antibody, DSTN antibody, dstn antibody, Dstn antibody, dstn.S antibody

Background:

Synonyms: 2610043P17Rik, ACTDP, Actin depolymerizing factor, Actin-depolymerizing factor, ADF, AU042046, bA462D18.2, corn1, DEST_HUMAN, Destrin actin depolymerizing factor, Destrin, DSN, Dstn, Sid 23, sid23p.

Background: Actin-depolymerizing factor (ADF), also known as destrin, is a member of the ADF/Cofilin/destrin superfamily that has the ability to rapidly depolymerize F-Actin in a stoichiometric manner. The Actin-depolymerizing activity of ADF is reversibly controlled by changes in KCl concentration but is insensitive to calcium concentration. ADF depolymerizes F-Actin by interacting directly with F-Actin protomers. ADF shares 71 % sequence homology with Cofilin, however the two proteins differ in their interaction with Actin. The difference in the function of ADF and Cofilin results from the subtle difference in their amino acid sequence rather than possible differences in posttranslational modifications. As a result of different cleavage sites on ADF and Cofilin, the proteins differ in their overall tertiary folds. Sensitivity to polyphosphoinositides may be a common feature in vitro among Actin-binding proteins such as ADF and Cofilin that can bind to G-Actin and regulate the state of Actin polymerization. ADF and Cofilin are Actin-depolymerizing proteins whose activities are possibly regulated by their phosphorylation/dephosphorylation.

Gene ID: 11034