ANAPC11 antibody (AA 1-84) (HRP)

Catalog No. ABIN1712104

Product Details anti-ANAPC11 Antibody (HRP)

Target: ANAPC11 (Anaphase Promoting Complex Subunit 11 (ANAPC11))

Binding Specificity: AA 1-84

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC11 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human APC11

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ANAPC11

Target: ANAPC11 (Anaphase Promoting Complex Subunit 11 (ANAPC11))

Alternative Name: APC11 (ANAPC11 Products)

Synonyms: APC11 antibody, Apc11p antibody, anapc11 antibody, RGD1561880 antibody, F10A16.17 antibody, F10A16_17 antibody, anaphase-promoting complex/cyclosome 11 antibody, DDBDRAFT_0187183 antibody, DDBDRAFT_0231277 antibody, DDB_0187183 antibody, DDB_0231277 antibody, 1110011I19Rik antibody, R75218 antibody, zgc:153671 antibody, si:dkey-237i9.4 antibody, anaphase promoting complex subunit 11 antibody, anaphase promoting complex subunit 11 L homeolog antibody, anaphase-promoting complex/cyclosome 11 antibody, anaphase-promoting complex subunit Apc11 antibody, ubiquitin-protein ligase Anaphase Promoting Complex antibody, hypothetical protein antibody, predicted protein antibody, anaphase-promoting complex subunit 11 antibody, APC11 anaphase promoting complex subunit 11 homolog (yeast) antibody, ANAPC11 antibody, anapc11.L antibody, anapc11 antibody, Anapc11 antibody, APC11 antibody, apc11 antibody, CAALFM_CR10610CA antibody, LOC9320604 antibody, LOC692923 antibody

Background:

Synonyms: ANAPC 11, ANAPC11, Anaphase promoting complex subunit 11, Anaphase promoting complex subunit 11 homolog yeast, Anaphase promoting complex subunit 11 homolog, Anaphase-promoting complex subunit 11, Apc 11, Apc 11p, APC11 anaphase promoting complex subunit 11 homolog yeast, APC11 anaphase promoting complex subunit 11 homolog, APC11, APC11_HUMAN, Apc11p, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, Hepatocellular carcinoma-associated RING finger protein, HSPC 214, HSPC214, MGC882, Yeast APC 11 homolog, Yeast APC11 homolog.

Background: Comprising more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to APC and activates APC?s cyclin-ubiquitination activity. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC11 is a RING-H2 finger protein that allows for the synthesis of multiubiquitin chains in the presence of Ubiquitin carrier protein 4 (Ubc4) and ubiquitin conjugating enzyme (E2). In addition, a heterodimeric complex of either Ubc4 or UbcH10 with APC11 and APC2 catalyzes the ubiquitination of human securin and cyclin B1.

Gene ID: 51529