GUCY1A1 antibody (AA 301-400) (HRP)

Catalog No. ABIN1712341

Product Details anti-GUCY1A1 Antibody (HRP)

Target: GUCY1A1 (Guanylate Cyclase 1 Soluble Subunit Alpha (GUCY1A1))

Binding Specificity: AA 301-400

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GUCY1A1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Guanylyl Cyclase alpha 1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GUCY1A1

Target: GUCY1A1 (Guanylate Cyclase 1 Soluble Subunit Alpha (GUCY1A1))

Abstract: GUCY1A1 Products

Synonyms: GC-SA3 antibody, GUC1A3 antibody, GUCA3 antibody, GUCSA3 antibody, GUCY1A1 antibody, 1200016O07Rik antibody, Gucy1a1 antibody, SGC antibody, im:7136149 antibody, sgc1a1 antibody, zgc:112159 antibody, gucy1a3 antibody, guanylate cyclase 1 soluble subunit alpha antibody, guanylate cyclase 1, soluble, alpha 3 antibody, guanylate cyclase 1 soluble subunit alpha 3 antibody, soluble guanylyl cyclase alpha subunit antibody, GUCY1A3 antibody, Gucy1a3 antibody, gucy1a3 antibody

Background:

Synonyms: GUCA3, MYMY6, GC-SA3, GUC1A3, GUCSA3, GUCY1A1, Guanylate cyclase soluble subunit alpha-3, GCS-alpha-3, GCS-alpha-1, Soluble guanylate cyclase large subunit, GUCY1A3

Background: Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase. The soluble forms, known as GCS or sGC, act as receptors for nitric oxide. The membrane-bound receptor forms, known as GC, are peptide hormone receptors. GCS, a cGMP-synthesizing enzyme, is the major receptor for the neurotransmitter nitric oxide (NO). It plays a crucial role in smooth muscle contractility, platelet reactivity and neurotransmission. GCS is a heme containing heterodimer, consisting of one alpha subunit, designated GCS-alpha-1, and one beta subunit. The heme moeity mediates NO activation, and this heme group also binds carbon monoxide, which weakly stimulates the enzyme. Both NO and CO stimulation are enhanced by the allosteric activator 3-(5'-hydroxymethyl-2'furyl)-benzyl-indazole, YC-1. YC-1 can also stimulate GCS in a NO-independent manner. Both the alpha and beta subunits are required for cGMP generation, and at least two isoforms exist for each subunit. Heterodimers consisting of alpha-1/beta-1 and alpha-2/beta-1 have been identified, and both display similar enzymatic activity.

Gene ID: 2982

UniProt: Q02108

Pathways: Myometrial Relaxation and Contraction