UBE2Z antibody (AA 151-259) (HRP)

Catalog No. ABIN1712406

Product Details anti-UBE2Z Antibody (HRP)

Target: UBE2Z (Ubiquitin-Conjugating Enzyme E2Z (UBE2Z))

Binding Specificity: AA 151-259

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2Z antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human USE1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UBE2Z

Target: UBE2Z (Ubiquitin-Conjugating Enzyme E2Z (UBE2Z))

Alternative Name: USE1 (UBE2Z Products)

Synonyms: USE1 antibody, AW049199 antibody, C030047H17Rik antibody, D11Moh35 antibody, RGD1308347 antibody, zgc:92419 antibody, Use1 antibody, ubiquitin conjugating enzyme E2 Z antibody, ubiquitin-conjugating enzyme E2Z antibody, ubiquitin conjugating enzyme E2Z S homeolog antibody, ubiquitin conjugating enzyme E2Z antibody, Ubiquitin-conjugating enzyme E2 Z antibody, UBE2Z antibody, Ube2z antibody, ube2z.S antibody, ube2z antibody, PTRG_07925 antibody, MCYG_07463 antibody, LOC580131 antibody, TERG_02129 antibody

Background:

Synonyms: 2010315L10Rik, 5730403H22Rik, AV002165, D12, Ed2, Embryonic development factor 2, MDS032, P31, Protein D12, Protein p31, putative MAPK activating protein PM26, Putative MAPK-activating protein PM26, Q-snare, RGD1306660, SLT1, SNARE-like tail-anchored protein 1 homolog, Unconventional SNARE in the ER 1 homolog S. cerevisiae, Unconventional SNARE in the ER 1 homolog, Use1, USE1 like protein, Use1 unconventional SNARE in the ER 1 homolog S. cerevisiae, USE1-like protein, USE1_HUMAN, USE1L, Vesicle transport protein USE1.

Background: In eukaryotic cells, the Golgi apparatus receives newly synthesized proteins from the endoplasmic reticulum (ER) and, after covalent modification, delivers them to their destination in the cell. For membrane-directed proteins this process is believed to be carried out via vesicular transport. Correct vesicular transport is determined by specific pairing of vesicle-associated SNAREs (v-SNAREs) with those on the target membrane (t-SNAREs). Unconventional SNARE in the ER 1, also known as USE1 or protein p31, is a 259 amino acid t-SNARE that forms a larger complex with ZW10, RINT-1 and Syntaxin 18. Upon Mg2+-AP treatment in the presence of NSF and ?SNAP, ZW10, RINT-1 and USE1 dissociate from Syntaxin 18. USE1 is a single-pass type IV membrane protein that is localized to the endoplasmic reticulum membrane. Three named isoforms exist for USE1 as a result of alternative splicing events.

Gene ID: 55850