SPAG9 antibody (AA 101-188)

Catalog No. ABIN1714030

Product Details anti-SPAG9 Antibody

Target: SPAG9 (Sperm Associated Antigen 9 (SPAG9))

Binding Specificity: AA 101-188

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAG9 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Sheep,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human JLP/JIP-4

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SPAG9

Target: SPAG9 (Sperm Associated Antigen 9 (SPAG9))

Alternative Name: JLP/JIP-4 (SPAG9 Products)

Synonyms: CT89 antibody, HLC-6 antibody, HLC4 antibody, JIP-4 antibody, JIP4 antibody, JLP antibody, PHET antibody, PIG6 antibody, 3110018C07Rik antibody, 4733401I23Rik antibody, 4831406C20Rik antibody, AW552012 antibody, JSAP2 antibody, JSAP2a antibody, Jip4 antibody, Mapk8ip4 antibody, syd1 antibody, sperm associated antigen 9 antibody, JNK-associated leucine-zipper protein antibody, C-Jun-amino-terminal kinase-interacting protein 4 antibody, SPAG9 antibody, Spag9 antibody, Bm1_41390 antibody, LOC100186844 antibody

Background:

Synonyms: C jun amino terminal kinase interacting protein 4, c Jun NH2 terminal kinase associated leucine zipper protein, FLJ13450, FLJ14006, FLJ34602, HLC 4, HLC 4 protein, HLC 6, HLC4, HLC4 protein, HLC6, HSS, Human lung cancer protein 6, Human lung cancer protein 6, JIP 4, JIP 4, JIP4, JNK associated leucine zipper protein 1, JNK associated leucine zipper protein, JNK interacting protein 4, JNK interacting protein 4, JNK interacting protein, JNK/SAPK associated protein, KIAA0516, Lung cancer oncogene 4, MAPK8IP4, Max binding protein, MGC117291, MGC14967, MGC74461, Mitogen activated protein kinase 8 interacting protein 4, Mitogen activated protein kinase 8 interacting protein 4, PHET, PIG 6, PIG6, proliferation inducing gene 6, Protein highly expressed in testis, SPAG 9, SPAG9, Sperm associated antigen 9, Sperm specic protein, Sperm surface protein, Sunday driver drosophila homolog, Sunday driver 1, SYD1, JIP4_HUMAN.

Background: JIP-4 is a 1,321 amino acid protein encoded by the human gene SPAG9. It contains a large N-terminal extracellular domain, a short transmembrane helical domain, and a cytoplasmic domain. There are 6 N-glycosylation sites, several phosphorylation sites for cAMP/cGMP-dependent protein kinase, protein kinase C, and casein kinase II, and 10 putative myristoylation sites. There is also a leucine zipper motif, with 6 leucine repeats, that may aid in dimerization since there is no upstream basic domain characteristic of DNA binding proteins. The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP-4 is a cytoplasmic, perinuclear protein that has eight known isoforms whose expression varies by tissue and disease state.

Gene ID: 9043

UniProt: O60271

Pathways: Regulation of Muscle Cell Differentiation