TRRAP antibody (AA 3401-3600)

Catalog No. ABIN1714098

Product Details anti-TRRAP Antibody

Target: TRRAP (Transformation/transcription Domain-Associated Protein (TRRAP))

Binding Specificity: AA 3401-3600

Reactivity: Human, Rat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRRAP antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Rat, Xenopus laevis

Predicted Reactivity: Mouse,Dog,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TRRAP

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for TRRAP

Target: TRRAP (Transformation/transcription Domain-Associated Protein (TRRAP))

Alternative Name: TRRAP (TRRAP Products)

Synonyms: PAF350/400 antibody, PAF400 antibody, STAF40 antibody, TR-AP antibody, Tra1 antibody, AI481500 antibody, TRRAP antibody, trrap antibody, transformation/transcription domain associated protein antibody, phosphotransferases/inositol or phosphatidylinositol kinase antibody, transcription-associated protein 1 antibody, transformation/transcription domain-associated protein antibody, TRRAP antibody, AT4G36080 antibody, LOC5577036 antibody, Trrap antibody, trrap antibody, LOC100637779 antibody

Background:

Synonyms: 350/400 kDa PCAF associated factor, FLJ10671, PAF 350/400, PAF 400, PAF350/400, PAF400, STAF 40, STAF40, TR AP, Tra 1, Tra1, Tra1 homolog, Transactivation / transformation domain associated protein, Transactivation/transformation domain associated protein, Transformation/transcription domain associated protein, TRAP, TRRAP_HUMAN.

Background: The transcription factors c-Myc and E2F are involved in regulating cell cycle progression. Overexpression of c-Myc in certain cell types induces noncycling cells to enter the cell cycle via a mechanism involving E2F-1 (1). E2F-1 is thought to regulate c-Myc expression via interactions with the retinoblastoma protein (2). TRRAP (for transformation/transcription domain-associated protein) interacts specifically with both c-Myc and E2F-1. Expression of trans-activated mutant TRRAP inhibits the oncogenic transformation of both c-Myc and E2F-1, suggesting that TRRAP is required for these oncogenic transcription factor pathways (3). TRRAP shares homology with the ATM/PI 3-kinase family, and it is highly conserved in evolution (4,5).

Gene ID: 8295