GCNT1 antibody (AA 201-260)

Catalog No. ABIN1714546

Product Details anti-GCNT1 Antibody

Target: GCNT1 (Glucosaminyl (N-Acetyl) Transferase 1, Core 2 (GCNT1))

Binding Specificity: AA 201-260

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCNT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GCNT1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GCNT1

Target: GCNT1 (Glucosaminyl (N-Acetyl) Transferase 1, Core 2 (GCNT1))

Alternative Name: GCNT1 (GCNT1 Products)

Synonyms: C2GNT antibody, C2GNT-L antibody, C2GNT1 antibody, G6NT antibody, NACGT2 antibody, NAGCT2 antibody, 5630400D21Rik antibody, B130048E03 antibody, C2 GlcNAcT antibody, IGnT antibody, core 2 GlcNAc-T antibody, glucosaminyl (N-acetyl) transferase 1, core 2 L homeolog antibody, glucosaminyl (N-acetyl) transferase 1, core 2 antibody, gcnt1.L antibody, GCNT1 antibody, Gcnt1 antibody

Background:

Synonyms: 3-galactosyl-O-glycosyl-glycoprotein beta-1, 6-N-acetylglucosaminyltransferase, Beta-1, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase, C2GNT, C2GNT L, C2GNT1, C2GnTL, Core 2 branching enzyme, Core 2 GNT, Core 2-branching enzyme, Core2 GlcNAc transferase, Core2-GlcNAc-transferase, GCNT 1, GCNT 1, GCNT1, GCNT1_HUMAN, glucosaminyl N-acetyl transferase 1 core 2, NACGT2.

Background: GCNT1 is expressed in a variety of cell types, including lymphocytes and mucin-producing cells. Specifically, GCNT1 expression in leukocytes regulates the synthesis of core 2 O-glycans that carry sialyl-Lewis x (sLex) oligosaccharides, which confer high affinity binding to Selectins. Downregulation of Selectin ligand expression levels has been shown to inhibit tissue infiltration. Therefore, GCNT1 represents a potential drug target for the treatment of inflammatory disorders and other pathologies involving Selectins.

Gene ID: 2650