V5 Epitope Tag antibody
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- Target See all V5 Epitope Tag products
- V5 Epitope Tag
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This V5 Epitope Tag antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Immunostaining (ISt)
- Sequence
- GKPIPNPLLG LDST
- Specificity
- Recognizes V5-tagged fusion proteins
- Sensitivity
- WB (with ECL): 1-2ng of V5-tagged fusion proteins
- Purification
- Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor
- Immunogen
- GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
- Clone
- E10
- Isotype
- IgG1
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- Application Notes
- WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
- Restrictions
- For Research Use only
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- Concentration
- 1mg/mL
- Buffer
- 10mM PBS, pH 7.2, 0.05% NaN3
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
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- Target
- V5 Epitope Tag
- Alternative Name
- V5-Tag (V5 Epitope Tag Products)
- Target Type
- Tag
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