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V5 Epitope Tag antibody

WB, ELISA, IP, DB, ISt Host: Mouse Monoclonal E10 unconjugated
Catalog No. ABIN1715158
  • Target See all V5 Epitope Tag products
    V5 Epitope Tag
    Reactivity
    Please inquire
    Host
    • 60
    • 41
    • 10
    • 7
    • 6
    • 2
    • 1
    Mouse
    Clonality
    • 57
    • 57
    • 10
    Monoclonal
    Conjugate
    • 66
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This V5 Epitope Tag antibody is un-conjugated
    Application
    • 101
    • 59
    • 44
    • 44
    • 31
    • 30
    • 13
    • 10
    • 8
    • 6
    • 6
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    • 1
    • 1
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Immunostaining (ISt)
    Sequence
    GKPIPNPLLG LDST
    Specificity
    Recognizes V5-tagged fusion proteins
    Sensitivity
    WB (with ECL): 1-2ng of V5-tagged fusion proteins
    Purification
    Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor
    Immunogen
    GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
    Clone
    E10
    Isotype
    IgG1
  • Application Notes
    WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
    Restrictions
    For Research Use only
  • Concentration
    1mg/mL
    Buffer
    10mM PBS, pH 7.2, 0.05% NaN3
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Target
    V5 Epitope Tag
    Alternative Name
    V5-Tag (V5 Epitope Tag Products)
    Target Type
    Tag
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