p300 antibody (AA 1572-2371)

Catalog No. ABIN363282

Product Details anti-p300 Antibody

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Binding Specificity: AA 1572-2371

Reactivity: Human, Mouse, Rat, Monkey, Mink

Host: Mouse

Clonality: Monoclonal

Conjugate: This p300 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Reacts with human p300 protein (residues 1868-1921)

Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Cross reacts with proteins from mouse, rat, monkey and mink.

Purification: Purified (protein A)

Immunogen: Fusion protein containing residues 1572-2371 of human p300

Clone: RW128

Isotype: IgG1 kappa

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ICC/IF1:10-1:2000, IP1:10-1:500, WB1:250-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, Sodium azide 0.1 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: p300 (EP300 Products)

Synonyms: KAT3B antibody, RSTS2 antibody, p300 antibody, A430090G16 antibody, A730011L11 antibody, p300 HAT antibody, E1A binding protein p300 antibody, histone acetyltransferase p300 antibody, EP300 antibody, LOC446056 antibody, ep300 antibody, Ep300 antibody

Background: Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.

Gene ID: 2033

UniProt: Q09472

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size