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PTPRF antibody (AA 1315-1607) (Atto 594)

PTPRF Reactivity: Human WB, ICC, IF Host: Mouse Monoclonal S165-38 Atto 594
Catalog No. ABIN1740985
  • Target See all PTPRF Antibodies
    PTPRF (Protein Tyrosine Phosphatase Receptor Type F (PTPRF))
    Binding Specificity
    • 20
    • 16
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1315-1607
    Reactivity
    • 56
    • 5
    • 5
    • 3
    • 1
    Human
    Host
    • 28
    • 26
    • 3
    Mouse
    Clonality
    • 32
    • 25
    Monoclonal
    Conjugate
    • 20
    • 5
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PTPRF antibody is conjugated to Atto 594
    Application
    • 33
    • 20
    • 18
    • 14
    • 13
    • 13
    • 11
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    Detects ~85 kDa (full length protein is 210 kDa - smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR. 97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK.
    Clone
    S165-38
    Isotype
    IgG2a
    Top Product
    Discover our top product PTPRF Primary Antibody
  • Application Notes
    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN1740985 was sufficient for detection of LAR/PTPRF in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    PTPRF (Protein Tyrosine Phosphatase Receptor Type F (PTPRF))
    Alternative Name
    LAR (PTPRF Products)
    Synonyms
    LAR antibody, AA591035 antibody, LARS antibody, RPTP-LAR antibody, LARFN5C antibody, zf-LAR antibody, protein tyrosine phosphatase, receptor type F antibody, protein tyrosine phosphatase, receptor type, F antibody, protein tyrosine phosphatase, receptor type, f, a antibody, PTPRF antibody, Ptprf antibody, ptprfa antibody
    Background
    PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule. PTPRF removes phosphate group from β-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function (1,2,3,4). Anti-PTPRF (C-terminal) antibody is specific for the extracellular and cytoplasmic subunits of human PTPRF (approx. 210, 150 and 85 kDa). Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.
    Gene ID
    5792
    UniProt
    P10586
    Pathways
    EGFR Signaling Pathway
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