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ATF4 antibody (AA 25-327) (FITC)

ATF4 Reactivity: Human WB, IHC, ICC, IF Host: Mouse Monoclonal S360A-24 FITC
Catalog No. ABIN1741065
  • Target See all ATF4 Antibodies
    ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))
    Binding Specificity
    • 25
    • 19
    • 16
    • 10
    • 10
    • 8
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 25-327
    Reactivity
    • 157
    • 56
    • 49
    • 23
    • 16
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Human
    Host
    • 138
    • 28
    • 1
    Mouse
    Clonality
    • 130
    • 37
    Monoclonal
    Conjugate
    • 78
    • 12
    • 9
    • 8
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This ATF4 antibody is conjugated to FITC
    Application
    • 130
    • 72
    • 41
    • 27
    • 26
    • 21
    • 19
    • 19
    • 13
    • 9
    • 6
    • 6
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    Detects ~60 kDa. Does not cross-react with ATF5.
    Cross-Reactivity
    Human, Rat
    Purification
    Protein G Purified
    Immunogen
    Fusion protein amino acids 25-327 of human ATF4. 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5.
    Clone
    S360A-24
    Isotype
    IgG2a
    Top Product
    Discover our top product ATF4 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN1741065 was sufficient for detection of ATF4 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))
    Alternative Name
    ATF4 (ATF4 Products)
    Synonyms
    CREB-2 antibody, CREB2 antibody, TAXREB67 antibody, TXREB antibody, ATF4 antibody, atf-4 antibody, creb-2 antibody, creb2 antibody, taxreb67 antibody, txreb antibody, atf4 antibody, wu:fj01c08 antibody, wu:fk30b04 antibody, zgc:85951 antibody, sb:eu681 antibody, wu:fb08f07 antibody, zgc:171702 antibody, Atf-4 antibody, C/ATF antibody, ATF4-I antibody, atf4-a antibody, atf4-b antibody, atf4-ii antibody, activating transcription factor 4 antibody, activating transcription factor 4 L homeolog antibody, activating transcription factor 4a antibody, activating transcription factor 4b antibody, activating transcription factor 4 S homeolog antibody, ATF4 antibody, atf4 antibody, Atf4 antibody, atf4.L antibody, atf4a antibody, atf4b antibody, atf4.S antibody
    Background
    Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation.
    Gene ID
    468
    NCBI Accession
    NP_001666
    UniProt
    P18848
    Pathways
    Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response
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