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Protocadherin gamma antibody (AA 808-931)

Reactivity: Mouse WB, IHC, ICC, IF Host: Mouse Monoclonal S159-5 unconjugated
Catalog No. ABIN1741179
  • Target See all Protocadherin gamma products
    Protocadherin gamma
    Binding Specificity
    AA 808-931
    Reactivity
    Mouse
    Host
    • 17
    Mouse
    Clonality
    • 17
    Monoclonal
    Conjugate
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Protocadherin gamma antibody is un-conjugated
    Application
    • 17
    • 10
    • 10
    • 10
    • 7
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    Detects ~100 kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids).
    Clone
    S159-5
    Isotype
    IgG1
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN1741179 was sufficient for detection of Protocadherin gamma (pan) in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Target
    Protocadherin gamma
    Abstract
    Protocadherin gamma Products
    Background
    The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
    Gene ID
    93709
    NCBI Accession
    NP_291062
    UniProt
    Q91XZ0
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