SNAP91 antibody (AA 279-297)

Catalog No. ABIN1742332

Product Details anti-SNAP91 Antibody

Target: SNAP91 (Synaptosomal-Associated Protein 91 (SNAP91))

Binding Specificity: AA 279-297

Reactivity: Human, Rat, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNAP91 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Specific for AP 180.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide (aa 279-297) of rat AP 180 coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-SNAP91 antibody (ABIN1742332)

Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Geis, Weishaupt, Hallermann, Grünewald, Wessig, Wultsch, Reif, Byts, Beck, Jablonka, Boettger, Üçeyler, Fouquet, Gerlach, Meinck, Sirén, Sigrist, Toyka, Heckmann, Sommer: "Stiff person syndrome-associated autoantibodies to amphiphysin mediate reduced GABAergic inhibition." in: Brain : a journal of neurology, Vol. 133, Issue 11, pp. 3166-80, (2010) (PubMed).

Zander, Münster-Wandowski, Brunk, Pahner, Gómez-Lira, Heinemann, Gutiérrez, Laube, Ahnert-Hilger: "Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 22, pp. 7634-45, (2010) (PubMed).

Target Details for SNAP91

Target: SNAP91 (Synaptosomal-Associated Protein 91 (SNAP91))

Alternative Name: AP 180 (SNAP91 Products)

Background: Synonyms: pp155, NP185, F1-20, AP-3, SNAP 91