PSMD8 antibody (AA 4-232)

Catalog No. ABIN1742386

Product Details anti-PSMD8 Antibody

Target: PSMD8 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8))

Binding Specificity: AA 4-232

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMD8 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Specific for p 31.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant human p 31 (aa 4-232).

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
ICC: 1 : 200
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-PSMD8 antibody (ABIN1742386)

Nieradka, Ufer, Thiadens, Grech, Horos, van Coevorden-Hameete, van den Akker, Sofi, Kuhn, von Lindern: "Grsf1-induced translation of the SNARE protein Use1 is required for expansion of the erythroid compartment." in: PLoS ONE, Vol. 9, Issue 9, pp. e104631, (2014) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Target Details for PSMD8

Target: PSMD8 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8))

Alternative Name: P 31 (PSMD8 Products)

Synonyms: HIP6 antibody, HYPF antibody, Nin1p antibody, Rpn12 antibody, S14 antibody, p31 antibody, hip6 antibody, hypf antibody, nin1p antibody, rpn12 antibody, psmd8b antibody, 6720456J22Rik antibody, AA407360 antibody, AL033291 antibody, AL033322 antibody, AL033323 antibody, C76433 antibody, psmd8 antibody, psmd8a antibody, fa93g07 antibody, fb17c09 antibody, fb49a10 antibody, zgc:86762 antibody, wu:fa93g07 antibody, wu:fb17c09 antibody, wu:fb49a10 antibody, PSMD8 antibody, proteasome 26S subunit, non-ATPase 8 antibody, proteasome 26S subunit, non-ATPase 8 S homeolog antibody, proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 antibody, proteasome 26S subunit, non-ATPase 8 L homeolog antibody, proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 pseudogene antibody, PSMD8 antibody, psmd8.S antibody, Psmd8 antibody, psmd8.L antibody, psmd8 antibody, LOC468490 antibody

Background: Synonyms: Use 1

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Proton Transport, Synthesis of DNA, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway