GABRA1 antibody (AA 28-43)
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- Target See all GABRA1 Antibodies
- GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
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Binding Specificity
- AA 28-43
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Reactivity
- Rat, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GABRA1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Specificity
- Specific for GABA-A receptor alpha1.
- Purification
- purified IgM. Azide was added before lyophilization.
- Immunogen
- Synthetic peptide (aa 28-43 of rat GABA-A receptor alpha1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
- Clone
- 53D3
- Isotype
- IgM
- Top Product
- Discover our top product GABRA1 Primary Antibody
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- Application Notes
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WB: 1 : 1000 up to 1 : 2000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500 - Comment
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ICC: This antibody is PFA fixation sensitive, use only mild fixation (2 % PFA). Best results are obtained by application of antibodies on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS, 0.02% sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- Storage
- -20 °C
- Storage Comment
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." in: Neuron, Vol. 91, Issue 4, pp. 792-807, (2016) (PubMed).
: "
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How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." in: Neuron, Vol. 91, Issue 4, pp. 792-807, (2016) (PubMed).
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- Target
- GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
- Alternative Name
- GABA-A Receptor Alpha1 (GABRA1 Products)
- Synonyms
- ECA4 antibody, EJM antibody, EJM5 antibody, zgc:153566 antibody, Gabra-1 antibody, gamma-aminobutyric acid type A receptor alpha1 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, alpha 1 antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 antibody, gamma-aminobutyric acid receptor subunit alpha-1 antibody, GABRA1 antibody, Gabra1 antibody, gabra1 antibody, LOC100653509 antibody
- Background
- Synonyms: GABA(A) R alpha1
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