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GABRA1 antibody (AA 28-43)

GABRA1 Reactivity: Rat, Mouse WB, IHC, ICC Host: Mouse Monoclonal 53D3 unconjugated
Catalog No. ABIN1742455
  • Target See all GABRA1 Antibodies
    GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
    Binding Specificity
    • 21
    • 20
    • 20
    • 20
    • 15
    • 15
    • 15
    • 14
    • 11
    • 11
    • 9
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 28-43
    Reactivity
    • 130
    • 106
    • 86
    • 14
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat, Mouse
    Host
    • 101
    • 73
    • 2
    Mouse
    Clonality
    • 101
    • 75
    Monoclonal
    Conjugate
    • 53
    • 12
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 9
    • 9
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    This GABRA1 antibody is un-conjugated
    Application
    • 166
    • 80
    • 75
    • 70
    • 33
    • 28
    • 26
    • 26
    • 17
    • 12
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Specificity
    Specific for GABA-A receptor alpha1.
    Purification
    purified IgM. Azide was added before lyophilization.
    Immunogen
    Synthetic peptide (aa 28-43 of rat GABA-A receptor alpha1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
    Clone
    53D3
    Isotype
    IgM
    Top Product
    Discover our top product GABRA1 Primary Antibody
  • Application Notes
    WB: 1 : 1000 up to 1 : 2000 (AP staining)
    IP: not tested yet
    ICC: 1 : 500
    IHC: 1 : 500
    Comment

    ICC: This antibody is PFA fixation sensitive, use only mild fixation (2 % PFA). Best results are obtained by application of antibodies on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    Buffer
    PBS, 0.02% sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
    Storage
    -20 °C
    Storage Comment
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Acuna, Liu, Südhof: "How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." in: Neuron, Vol. 91, Issue 4, pp. 792-807, (2016) (PubMed).

  • Target
    GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
    Alternative Name
    GABA-A Receptor Alpha1 (GABRA1 Products)
    Synonyms
    ECA4 antibody, EJM antibody, EJM5 antibody, zgc:153566 antibody, Gabra-1 antibody, gamma-aminobutyric acid type A receptor alpha1 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, alpha 1 antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 antibody, gamma-aminobutyric acid receptor subunit alpha-1 antibody, GABRA1 antibody, Gabra1 antibody, gabra1 antibody, LOC100653509 antibody
    Background
    Synonyms: GABA(A) R alpha1
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