GABRB1 antibody (AA 342-430)

Catalog No. ABIN1742466

Product Details anti-GABRB1 Antibody

Target: GABRB1 (gamma-aminobutyric Acid (GABA) A Receptor, Subunit beta 1 (GABRB1))

Binding Specificity: AA 342-430

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Specific for GABA-A receptor beta1.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein (aa 342-430 in mouse) of the unprocessed precursor forced into a loop-shaped structure.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500

Comment:

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-GABRB1 antibody (ABIN1742466)

Kerti-Szigeti, Nusser: "Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells." in: eLife, Vol. 5, (2016) (PubMed).

Target Details for GABRB1

Target: GABRB1 (gamma-aminobutyric Acid (GABA) A Receptor, Subunit beta 1 (GABRB1))

Alternative Name: GABA-A Receptor Beta1 (GABRB1 Products)

Synonyms: AW061132 antibody, B230208N19Rik antibody, Gabrb-1 antibody, GARB1 antibody, si:dkey-20i10.4 antibody, gamma-aminobutyric acid type A receptor beta1 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, subunit beta 1 antibody, gamma-aminobutyric acid type A receptor beta 1 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, beta 1 antibody, GABRB1 antibody, Gabrb1 antibody, gabrb1 antibody

Background: Synonyms: GABA(A) R beta1