NR5A2 + LRH1 antibody (Internal Region)

Catalog No. ABIN1781851

This Goat Polyclonal antibody specifically detects NR5A2 + LRH1 in WB and ELISA. It exhibits reactivity toward Human and Pig.

Quick Overview for NR5A2 + LRH1 antibody (Internal Region) (ABIN1781851)

Target: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Reactivity: Human, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This NR5A2 + LRH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-NR5A2 + LRH1 Antibody

Binding Specificity: Internal Region

Purpose: NR5A2 / LRH1

Sequence: TDYDRSPFVT SPIS

Specificity: This antibody is expected to recognize both reported isoforms (NP_995582.1, NP_003813.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in nuclear lysates of cell lines HeLa and HepG2, and approx 60 kDa band observed in Pig Ovary lysates (calculated MW of 56.5 kDa according to Human NP_003813.1 and Pig AFD98842.1). Recommended concentration: 0.3-1 μg

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NR5A2 + LRH1

Target: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Alternative Name: NR5A2

Background: NR5A2, nuclear receptor subfamily 5, group A, member 2, B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2, CYP7A promoter-binding factor, alpha-1-fetoprotein transcription factor, b1-binding factor, hepatocyte transcription factor which activat

Molecular Weight: Expected molecular weight: 55 kDa

Gene ID: 2494, 26424, 60349

NCBI Accession: NP_995582, NP_003813

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway