Fibrinogen beta Chain antibody (AA 177-190)

Catalog No. ABIN1782152

Product Details anti-Fibrinogen beta Chain Antibody

Target: Fibrinogen beta Chain (FGB)

Binding Specificity: AA 177-190

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Fibrinogen beta Chain antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: fibrinogen beta chain (aa177-190)

Sequence: EKHQLYIDET VNSN

Specificity: This antibody is expected to recognize both reported isoforms (NP_005132.2, NP_001171670.1).

Cross-Reactivity: Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta and Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in Human Liver lysates (calculated MW of 55.9 kDa according to NP_005132.2). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig Liv

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Fibrinogen beta Chain

Target: Fibrinogen beta Chain (FGB)

Alternative Name: FGB (FGB Products)

Background: FGB, fibrinogen beta chain, fibrinogen, B beta polypeptide

Molecular Weight: Expected molecular weight 60 kDa

Gene ID: 2244

NCBI Accession: NP_005132, NP_001171670