CD209b Antigen (CD209B) antibody

Catalog No. ABIN1106055

Product Details

Target: CD209b Antigen (CD209B)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises SIGN-R1, a C-type lectin that has been identified as a murine homologue of the human DC-SIGN related molecule, L-SIGN. Mouse SIGN-R1 is specifically expressed on marginal zone macrophages in the spleen, medullary and subcapsular sinus macrophages in the lymph nodes, and at lower levels on liver sinusoidal endothelial cells. SIGN-R1 has not been detected on murine dendritic cell populations. Like many C-type lectins, SIGN-R1 functions as a pathogen recognition receptor in vivo and plays an important role in defence against pathogens. Antibodies, produced by clone ER-TR9, are reported to block the uptake of neutral polysaccharides by macrophages.

Purification: Ammonium sulphate precipitation

Endotoxin Level: Low Endotoxin

Immunogen: Isolated C3H thymic stromal cells. - Spleen cells from immunised rats were fused with cells of the Mouse P3-X63-Ag8.563 myeloma cell line.

Clone: ER-TR9

Isotype: IgM

Application Details

Application Notes: Flow Cytometry: 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/25 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.4

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C only.

References

Weisel, Appelt, Schneider, Horlitz, van Rooijen, Korner, Mach, Winkler: "Unique requirements for reactivation of virus-specific memory B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4011-21, (2010) (PubMed).

Target Details

Target: CD209b Antigen (CD209B)

Alternative Name: CD209b / DC-SIGNR1 (CD209B Products)

Background: SIGN R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. MZM of the spleen are involved in the clearance of polysaccharides. Mouse SIGN R1 is a C type lectin, like DC SIGN which is expressed on Human dendritic cells (DCs). However, Mmouse SIGN R1 itself is not expressed on DCs. SIGN R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides.Synonyms: CD209 antigen-like protein B, DC-SIGN-related protein 1, OtB7

Gene ID: 69165

NCBI Accession: NP_001032889

UniProt: Q8CJ91