IRS4 antibody (AA 633-647)

Catalog No. ABIN1833992

Product Details anti-IRS4 Antibody

Target: IRS4 (Insulin Receptor Substrate 4 (IRS4))

Binding Specificity: AA 633-647

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IRS4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Mouse IRS4

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DRGTKERKEAKEVRD from an internal region of mouse IRS4 (NP_034702.2). Percent identity by BLAST analysis: Mouse (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:128000), WB (0.2 - 0.6 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 150kD band observed in Mouse Heart lysates (calculated MW of 130kD according to NP_034702.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreyer et al, jEndocrinology. 2003 Apr,144(4):1211-8. PMID: 12639902). Recommended concentration: 0.2-0.6 μg/mL. An additional band of unknown identity was also consistently observed at ~39kD. This band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IRS4

Target: IRS4 (Insulin Receptor Substrate 4 (IRS4))

Alternative Name: IRS4 (IRS4 Products)

Synonyms: IRS4 antibody, irs-4 antibody, py160 antibody, IRS-4 antibody, PY160 antibody, irs2 antibody, irs2-a antibody, insulin receptor substrate 4 antibody, insulin receptor substrate 4 S homeolog antibody, IRS4 antibody, irs4 antibody, Irs4 antibody, irs4.S antibody

Background: Name/Gene ID: IRS4

Synonyms: IRS4, Insulin receptor substrate 4, Phosphoprotein of 160 kDa, IRS-4, Pp160, PY160

Gene ID: 8471

NCBI Accession: NP_034702

UniProt: O14654