Influenza Hemagglutinin HA1 Chain antibody (Influenza A Virus H1N1 (A/Puerto Rico/8/1934)) (AA 200-250)

Catalog No. ABIN1841885

Product Details

Target:

Influenza Hemagglutinin HA1 Chain (HA1)

Binding Specificity: AA 200-250

Reactivity: Influenza A Virus H1N1

Virus Strain: A/Puerto Rico/8/1934

Host: Rabbit

Clonality: Polyclonal

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Specificity: Specific for HA1.

Purification: Whole serum

Immunogen: Synthetic peptide from aa region 200-250 of Influenza A virus conjugated to an immunogenic carrier protein was used as the antigen.

Application Details

Application Notes: A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details

Target:

Influenza Hemagglutinin HA1 Chain (HA1)

Synonyms: hemagglutinin, Hemagglutinin, HA, ha

Target Type: Influenza Protein

Background: Function: Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Subunit structure: Homotrimer of disulfide-linked HA1-HA2. Subcellular location: Virion membrane, Single-pass type I membrane protein. Host apical cell membrane, Single-pass type I membrane protein. Note: Targeted to the apical plasma membrane in epithelial polarized cells through a signal present in the transmembrane domain. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts. Miscellaneous: Major glycoprotein, comprises over 80% of the envelope proteins present in virus particle.
Synonyms: Hemagglutinin, HA