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His Tag antibody (Biotin)

WB Host: Rabbit Polyclonal Biotin
Catalog No. ABIN1842272
  • Target See all His Tag Antibodies
    His Tag
    Reactivity
    Please inquire
    Host
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    Rabbit
    Clonality
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    Polyclonal
    Conjugate
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    This His Tag antibody is conjugated to Biotin
    Application
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    Western Blotting (WB)
    Sequence
    HHHHHH
    Specificity
    His-tag antibody [Biotin], pAb, Rabbit specifically reacts with fusion proteins containing His tagged fusion proteins.
    Characteristics
    His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody. It is suitable for detecting the expression level of His tagged proteins. His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody. The antibody recognizes fusion protein with His tag which is located at either C-terminal, or internal regions of the protein. It is suitable for detecting the expression level of His tagged proteins.
    Purification
    Immunoaffinity chromatography
    Immunogen
    His epitope tag peptide HHHHHH conjugated to KLH.
    Isotype
    IgG
    Top Product
    Discover our top product His Tag Primary Antibody
  • Application Notes
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    Western blot: 0. 2-1 µg/mL
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
    Concentration
    0.5 mg/mL
    Buffer
    lyophilized with PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
  • Dong, Blissard: "Functional analysis of the Autographa californica multiple nucleopolyhedrovirus GP64 terminal fusion loops and interactions with membranes." in: Journal of virology, Vol. 86, Issue 18, pp. 9617-28, (2012) (PubMed).

  • Target
    His Tag
    Abstract
    His Tag Products
    Target Type
    Tag
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